中国耳鼻咽喉头颈外科2016，Vol.23Issue(4)：211-215,5.DOI:10.16066/j.1672-7002.2016.04.007
Wnt/β-catenin信号通路调控下游基因CD44对鼻咽癌侧群细胞的作用和意义
Effect and its significance of Wnt/β-catenin signaling pathway by controlling tumor marker CD44 on nasopharyngeal carcinoma SP cells
摘要
Abstract
[ABSTRACT]OBJECTIVEThis study aims to explore the molecular mechanism and expression of Wnt/β-catenin signaling pathway and tumor marker CD44 in nasopharyngeal carcinoma cells after transfection withβ-Catenin when the Wnt/β-catenin signaling pathway was blocked.METHODSSP cells and CD44+SP cells isolated from the nasopharyngeal carcinoma cell line CNE-2 by flow cytometry were identified. Changes in the number and biological characteristics of CNE-2 and CD44+SP cells in vitro were investigated after the Wnt/β-catenin signaling pathway was blocked through siRNA.RESULTSSP cells accounted for 2.3% of nasopharyngeal carcinoma CNE-2 cells, andCD44+SP cells accounted for 36.5% of the SP cells. CD44+SP cells showed significantly higher in vitro proliferation and resistance to chemotherapy (P<0.05). After transfection withβ-Catenin siRNA, the proliferation, cloning efficiency, and tolerance to chemotherapeutic drugs of the cells were found statistical differences compared with those before transfection ofβ-Catenin siRNA. CONCLUSIONWnt/β-catenin signaling pathway abnormalities are closely related to the biological behavior of nasopharyngeal carcinomaCD44+SP cells. Interference of this pathway can change the characteristics of nasopharyngeal carcinoma stem cells.关键词
鼻咽肿瘤/肿瘤干细胞/流式细胞术/细胞周期/Wnt/β-catenin信号通路/CD44/肿瘤标志物Key words
Nasopharyngeal Neoplasms/Tumor Stem Cell/Flow Cytometry/Cell Cycle/wnt/β-catenin signaling pathway/CD44/tumor marker引用本文复制引用
熊姗姗,邱前辉,罗小宁,崔勇,苏小妹,许咪咪..Wnt/β-catenin信号通路调控下游基因CD44对鼻咽癌侧群细胞的作用和意义[J].中国耳鼻咽喉头颈外科,2016,23(4):211-215,5.基金项目
广东省科技计划项目 （）
