中国耳鼻咽喉头颈外科2016，Vol.23Issue(5)：250-252,3.DOI:10.16066/j.1672-7002.2016.05.002
甲状腺髓样癌家系RET基因点突变质粒及稳定细胞系构建
Plasmid and stable cell linereconstruction of RET point mutation in medullary thyroid carcinoma pedigree
陆凡倩 1陈晓红 1寇秀娟 1白云龙 1冯亚茹 1杨静 1王伯淳1
作者信息
- 1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科，耳鼻咽喉头颈科学教育部重点实验室 首都医科大学，北京 100730
- 折叠
摘要
Abstract
OBJECTIVEThe plasmid construction and validation of RET point mutation in vitro.METHODS The RET gene exon in the mainland familial medullary thyroid carcinoma family was analized with muon capture "two generation" sequencing of. In vitro, the RET relative point mutationswere reconstructed in NIH3T3 cells and the expression levels were studied.RESULTSThe corresponding lentiviral plasmids of RET point mutations were successfully obtained after point mutating the wild RET plasmids, it was verified that the target genes were expressed in NIH3T3 cells stably by Western Blot. CONCLUSIONSStable cell lines carrying RET point mutations were reconstructedsuccessfully, which provide a good platform for studying various point mutations.关键词
甲状腺肿瘤/基因/突变/质粒/慢病毒感染/细胞系/信号通路Key words
Thyroid Neoplasms/Genes/Mutation/Plasmids/Lentivirus Infections/Cell Line/signaling pathway引用本文复制引用
陆凡倩,陈晓红,寇秀娟,白云龙,冯亚茹,杨静,王伯淳..甲状腺髓样癌家系RET基因点突变质粒及稳定细胞系构建[J].中国耳鼻咽喉头颈外科,2016,23(5):250-252,3.
