中国耳鼻咽喉头颈外科2016，Vol.23Issue(5)：259-262,4.DOI:10.16066/j.1672-7002.2016.05.004
染料木黄酮增强喉癌Hep-2细胞放射敏感性的研究
Radiosensitizing effect of genistein on laryngeal carcinoma Hep-2 cells
王惠民 1周渝 2张淑香3
作者信息
- 1. 武警后勤学院附属医院耳鼻咽喉头颈外科，天津300162
- 2. 河北医科大学研究生学院，河北 石家庄050017
- 3. 武警杭州士官学校校务部门诊部，浙江 杭州300023
- 折叠
摘要
Abstract
OBJECTIVETo investigate whether the genistein can increase the radiosensitizing effect on laryngeal squamous carcinoma Hep-2 cells.METHODS Hep-2 cells were treated with genistein, radiation, and genistein plus radiation respectively. DMSO was used as the control group. EdU assay was performed to assess the short-term effect of genistein and (or) radiation on the proliferation of Hep-2 cells. Clonegenic assay was used to detect the survival rate of Hep-2 cells after treatment with radiation doses of 0, 2, 4, 6, 8 Gy and radiation combined with genistein. The data was fitted into the classic single-hit multi-target mathematical model to analyze the long-term effect on cell proliferation death of Hep-2 cells.RESULTSIt was observed that radiation combined with genistein could significantly inhibit the proliferation of Hep-2 cells. And the SER of 10μmol/L genistein was 1.412.CONCLUTIONGenistein can inhibit the proliferation of Hep-2 cells by DNA synthesis inhibition, and can be an adjunct agent of radiotherapy.关键词
辐射耐受性/肿瘤细胞,培养的/木黄酮Key words
Radiation Tolerance/Tumor Cells, Cultured/genistein引用本文复制引用
王惠民,周渝,张淑香..染料木黄酮增强喉癌Hep-2细胞放射敏感性的研究[J].中国耳鼻咽喉头颈外科,2016,23(5):259-262,4.
