福建农业学报2016，Vol.31Issue(4)：350-355,6.DOI:10.19303/j.issn.1008-0384.2016.04.004
茉莉花 J sGGP PS 基因的克隆及生物信息学与表达分析
Cloning,Molecular Characterization,and Expression of JsGGPPs Gene from Jasminum sambac
摘要
Abstract
Geranylgerany pyrophosphate synthase (GGPPS ) catalyzes the biosynthesis of geranylgeranyl diphosphate,which is a key precursor of the aromatic terpenes in jasmine flowers.The full-length cDNA sequence of GGP PS gene in the terpenoid backbone biosynthesis was cloned from J asminum sambac by RT-PCR combined with RACE,and subsequently named J sGGP PS with a GenBank Accession number of AIY24421.1. The expression of J sGGP PS during jasmine blooming was detected by quantitative real-time PCR.The results showed that the full-length of the cDNA sequence was 1 410 bp with a 1 083 bp open reading frame.The deduced protein consisted of 360 amino acids,and shared 91% identity with GGPPS from Lepidium apetalum and 84% from Taraxacum kok-saghyz .J sGGP PS had one chain length determination region,two highly conserved aspartate-rich motifs,and other conserved domains,which belonged to the trans-isoprenyl diphosphate synthases and class I terpene cyclases superfamily.The quantitative real-time PCR showed that the J sGGP PS expression at 18:00 was low,and began to rise and reached a maximum at 20:00 followed by a steady decline to a minimum at 2:00.The observation significantly correlated with the aroma release pattern of J.sambac in nature.The results provided a basis for further study on the terpene biosynthetic pathway of the flower.关键词
茉莉花/萜烯类/香气/牻牛儿基牻牛儿基焦磷酸合酶/克隆/表达分析Key words
Jasmimun sambac/terpene/fragrance/geranylgerany pyrophosphate synthase/cloning/expression analysis分类
农业科技引用本文复制引用
孙君,林浥,俞滢,陈静,姚雪倩,陈桂信,叶乃兴..茉莉花 J sGGP PS 基因的克隆及生物信息学与表达分析[J].福建农业学报,2016,31(4):350-355,6.基金项目
福建省自然科学基金(2016J01110) （2016J01110）
福州市农业局2014年福州茉莉花茶产业提升项目(2014-3) （2014-3）
