福建农业学报2016，Vol.31Issue(4)：377-383,7.DOI:10.19303/j.issn.1008-0384.2016.04.009
小菜蛾气味结合蛋白 PxylOBP13基因克隆与序列分析
Cloning and Sequence Analysis of an Odorant Binding Protein Gene PxylOBP 13 in the Diamondback Moth,Plutella xylostella (Lepidoptera:Plutellidae)
摘要
Abstract
The diamondback moth (DBM),P lutella xylostella (L.)is a key insect pest on cruciferous plants all over the world. Olfactory perception is critical for survival and reproduction in insects,and correspondingly provides a new target to control DBM. Hereby,an odorant binding protein (OBP)gene from P.xylostella,PxylOBP 13 (GenBank accession number:KT1 56679 )was cloned;the bio-information characteristics were analyzed;and a phylogenetic tree was constructed. The results showed that the full-length of open reading frame of PxylOBP 13 is 387 bp and encodes 128 amino acids;PxylOBP 13 contains 6 conserved cysteine residues and the conserved domain of odorant binding protein family,and 3 hydrophobic regions which are potential binding sites for hydrophobic odorants. The protein molecular mass was predicted as 14.33 kDa with an isoelectric point of 7.55,but the N-terminal without signal peptide;the similarity of sequence between PxylOBP 13 and six lepidopteran insects can reach above 50%. The results can provide theoretical support for further research on the function of OBPs in DBM and development of effective management of DBM based on olfactory mechanism.关键词
小菜蛾/气味结合蛋白/克隆/序列分析Key words
Plutella xylostella/odorant binding protein/cloning/sequence analysis分类
生物科学引用本文复制引用
覃江梅,程小娟,魏辉,蔡立君,尤民生..小菜蛾气味结合蛋白 PxylOBP13基因克隆与序列分析[J].福建农业学报,2016,31(4):377-383,7.基金项目
国家自然科学基金项目(31301666、31230061、31320103922) （31301666、31230061、31320103922）
福建省自然科学基金(2014J05031) （2014J05031）
