福建农业学报2016，Vol.31Issue(4)：419-423,5.DOI:10.19303/j.issn.1008-0384.2016.04.016
暗棕壤性苹果梨园土壤有机磷组分提取流程的优化
Optimizing Extraction Process Organic Phosphorus Fractionation from Dark Brown Soil at Apple-pear Orchards
摘要
Abstract
Dark brow soil at the apple-pear orchards at Yanji in the Korean Autonomous Prefecture of Yanbian in Jilin province was sampled for the experimentation on organic phosphorus fractionation.The process was optimized by using the Bowman-Cole method modified by Xiong Heng-duo and Fan Ye-kuan.The fractionation included the following major steps:(1)to the acidic soil pretreated with chloroform,0.5 mol·L-1 of NaHCO3 were added with ultrasonic treatment for 10 minutes followed by oscillation for 30 minutes to extract the labile organic phosphorus into the supernatant;(2 )to the soil left after the initial extraction,0.1 mol · L-1 of NaOH was added with ultrasound for 10 minutes followed by oscillating for 4 hours to leach the stable and the moderately labile organic phosphorus fractions into the alkali solution;(3)to the above alkali supernatant,acid was added to adjust the pH to 3.00 to precipitate the highly stable organic phosphorus and allow the moderately stable fraction remain in the solution;and finally,(4)to the soil,1 mol·L-1 of H 2 SO4 was added with a constant oscillation for 3 hours to extract the moderately labile organic phosphorus.In comparison to the existing methods,the current fractionation process on the acidic orchard soil in north China appeared to have optimized the conditions with improved efficiency.关键词
苹果梨园/土壤/有机磷Key words
apple-pear orchard/soil/organic phosphorus分类
农业科技引用本文复制引用
窦文涛,王非非,谢修鸿,程晶,梁运江..暗棕壤性苹果梨园土壤有机磷组分提取流程的优化[J].福建农业学报,2016,31(4):419-423,5.基金项目
国家自然科学基金项目(31460117) （31460117）
延边大学第7届本科生科研立项项目(ydbksky2015052) （ydbksky2015052）
