口腔疾病防治2016，Vol.24Issue(6)：336-340,5.DOI:10.12016/j.issn.2096-1456.2016.06.004
shRNA沉默LASP-1对舌鳞状细胞癌SCC9细胞增殖和迁移的影响
Influence of shRNA silent LASP-1 on cell proliferation and migration of tongue squamous cell carcinoma
摘要
Abstract
Objective To investigate the expression of LASP⁃1 in tongue squamous cell carcinoma, and to study the influence of shRNA silent LASP⁃1 on cell proliferation and migration of tongue squamous cell carcinoma. Methods Green fluorescence shRNA targeting LASP⁃1 was constructed and transfected into SCC9 cells by lipofectamine. Cell proliferation was detected by MTT method. LASP⁃1 mRNA and protein were determined by RT⁃PCR and western blot respectively. The migration ability of cells was detected by transwell assay. Results There was green fluorescence ex⁃pression in experimental and negative control group cells after transfecting shRNA and shNC respectively. LASP⁃1 mRNA and protein were decrease in experimental group cells. It indicated that LASP⁃1 shRNA was transfected success⁃fully. By contrast with control group, the cell viability of experimental group cells reduced by (51.23 ± 1.47)% and (50.07 ± 2.11)% after 48 h and 72 h respectively. The results of transwell assay showed that migration ability of SCC9 cells was decreased significantly after shRNA targeting LASP⁃1 was successful transfected. It decreased by 43% com⁃pared with control group. Conclusion LASP⁃1 has high expression in tongue squamous cell carcinoma cells, down reg⁃ulated LASP⁃1 gene expression can inhibit the proliferation and migration of SCC9 cells.关键词
LASP-1/shRNA/舌鳞状细胞癌/细胞增殖/迁移Key words
LASP-1/shRNA/Tongue squamous cell carcinoma/Cell proliferation/Migration分类
医药卫生引用本文复制引用
于丽娜,张文轩,于洋,秦文光,郭雪琪..shRNA沉默LASP-1对舌鳞状细胞癌SCC9细胞增殖和迁移的影响[J].口腔疾病防治,2016,24(6):336-340,5.基金项目
广东省自筹经费类科技计划项目 （）
