化工学报2016，Vol.67Issue(6)：2503-2509,7.DOI:10.11949/j.issn.0438-1157.20151778
类泛素介导和热激响应协同提高酿酒酵母的热稳定性
Synergistically enhanced thermostability ofSaccharomyces cerevisiaeby ubiquitin-like protein mediation and heat shock response
摘要
Abstract
To improve the thermostability and fermentation performance ofSaccharomyces cerevisiae to reduce the energy consumption of the cooling progress in industrial fermentation, the protein homeostasis was regulated through ubiquitin-like protein mediation and heat shock response. In this study, many heat-resistant gene devices were mined out from genes related to protein homeostasis and constructed with the regulatory device FBA1p, and then transformed intoSaccharomyces cerevisiae INVSC1. Outstanding heat-resistant devices FBA1p-atg8 and FBA1p-hsp104 were screened through gradually increased temperature incubation. Compared with the control, the OD660 of the engineered yeast strainsS.c-ATG8 and S.c-HSP104 were both over 50% higher (84 h) and their cell viability were 1.64 to 3.01 times higher (72 h) when cultured at 40℃. The physiological characteristics implied that the thermotolerant strains possessed better cell wall integrity and higher trehalose content. In order to strengthening the regulatory mechanisms of both ubiquitin-proteasome system pathway and heat-shock responses within the network of protein homeostasis, atg8 andhsp104were assembled to construct bifunctional engineered strainS.c-ATG8-HSP104, which showed better growth ability, stronger cell activity and higher ethanol yield at 40℃. The results revealed that the synergistic effect of ubiquitin-like protein and heat shock protein could enhance yeast thermotolerance and improve strain activity.关键词
合成生物学/发酵/生物技术/类泛素/热激蛋白/蛋白质平衡/热稳定性Key words
synthetic biology/fermentation/biotechnology/ubiquitin-like protein/heat shock protein/protein homeostasis/thermostability分类
化学化工引用本文复制引用
肖冰,李珺,李春..类泛素介导和热激响应协同提高酿酒酵母的热稳定性[J].化工学报,2016,67(6):2503-2509,7.基金项目
国家自然科学基金项目（21376028，21576027）；国家杰出青年科学基金项目（21425624）。@@@@supported by the National Natural Science Foundation of China (21376028,21576027) and the Science Fund for Distinguished Youth Scholars of China (21425624) （21376028,21576027）
