湖南中医药大学学报2016，Vol.36Issue(6)：11-15,5.DOI:10.3969/j.issn.1674-070X.2016.06.004
加味丹参饮预处理对大鼠心肌缺血再灌注损伤的保护作用
Protective Effects of Modified Danshen Decoction Pretreatment on Myocardial Ischemia Reperfusion Injury in Rats
摘要
Abstract
〔Abstract〕 Objective To investigate the protective effects of Jiawei Danshen decoction (JDSH) pretreatment on myocardial ischemia reperfusion injury (IRI) in rats and its molecular mechanisms. Methods The models of left anterior descending coronary artery of ligatured rats for 30 mins and then I/R 30 (or I/R 60) mins were built, fifty-six male SD rats were randomly divided into 7 groups: sham operation group, ischemia reperfusion (I/R) injury for 30 min group (I/R 30),I/R for 60 mins (I/R 60) group, p38MAPK inhibitor SB203580 with I/R30 mins (SB203580+I/R30) group, SB203580+I/R 60 group, JDSH+I/R 30 group, JDSH+I/R 60 group. After intervention for two days, the myocardial tissue was observed with HE Dyeing, the serum CK and LDH were measured by the automatic biochemical analyzer, the expression of p38MAPK, COX-2, ICAM-1 were determined by immunohistochemical assay. Results The cardiomyocytes' morphological structure with JDSH or p38MAPK inhibitor SB203580 pretreatment was better. Compared with sham operation group, the content of CK and LDH in serum of I/R 30 and I/R 60 groups was increased obviously (P<0.01). Compared with I/R 30 and I/R60 groups, the SB 203580+I/R30 or 60 and JDSH+I/R30 or60 groups were reduced distinctly (P<0.01). Compared with sham operation group, the myocardial tissue p38MAPK, COX-2, ICAM-1 protein expression in I/R rats were increased (P<0.01), and increased with the extension of reperfusion time. Compared with I/R 30/60 groups, the protein expression in SB203580 and JDSH groups was decreased (P<0.01). Conclusion Myocardial IRI in rats activates the p38MAPK signaling pathway which is positively correlated with reperfusion time (30 min to 60 min). JDSH can alleviate the myocardial damage by inhibition of the p38MAPK signaling pathway, reducing the expression of the COX-2 and ICAM-1, and CK, LDH, to protect ischemic heart muscle.关键词
加味丹参饮/心肌/缺血再灌注/p38MAPK/COX-2/ICAM-1/丹参/檀香/赤芍Key words
modified Danshen decoction/myocardium/Ischemia reperfusion/p38MAPK/COX-2/ICAM-1/Salvia miltiorrhiza Bge./Santalum album L./Paeonia veitchii Lynch分类
医药卫生引用本文复制引用
陈聪,任婷,胡华,黄政德,廖菁..加味丹参饮预处理对大鼠心肌缺血再灌注损伤的保护作用[J].湖南中医药大学学报,2016,36(6):11-15,5.基金项目
国家自然科学基金资助项目（81373576，81503536)；湖南省教育厅科学研究项目（14C0872，14B136）；湖南省科技厅科学研究项目（2014SK3034）；湖南省中医药科研计划课题（B0347）；中西医结合防治心脑疾病的相关基础研究湖南省高校科技创新团队科研项目（5）；中医内科学省部共建教育部重点实验室开放基金重点项目（ZYNK201401）。 （）
