湖南中医药大学学报2016，Vol.36Issue(6)：24-28,5.DOI:10.3969/j.issn.1674-070X.2016.06.007
不同浓度的烟焦油与尼古丁对脐静脉内皮细胞增殖作用的研究
Study of Cigarette Tar and Nicotine with Different Concentrations on the Proliferation of Human Umbilical Vein Endothelial Cells
摘要
Abstract
〔Abstract〕 Objective To observe the proliferation of human umbilical vein endothelial cells (HUVECs) under different tar and nicotine conditions. Methods HUVECs were isolated by type I collagenase digestion. After passage cultivation, we used the factor Ⅷ related antibody to identify endothelial cell. CCK-8 assay and BrdU staining were used to estimate the effect of tar and nicotine on proliferation of HUVECs. Results 1. Ⅷ related antibody staining showed that more than 95%were endothelial cells in cultured cells. 2. A values at 450 nm of 50 mg/mL tar group were reduced than the control group after culturing for 6 h (P<0.05). A value of 50 mg/mL tar group were reduced compared with any other groups after culturing for 24 h (P<0.05). With the increasing of nicotine concentration, A values were gradually increased in nicotine groups compared with control group. However, there were no significant differences. 3. BrdU positive rates of HUVECs had no significant difference between tar groups compared with control group after cultivation for 6 h. The BrdU positive rate of50 mg/mL tar group were reduced comparing with any other groups after culturation for 24 hours (P<0.05). There was no significant difference of BrdU positive rate between nicotine groups and control group after cultivation for 6 h and 24 h. Conclusion The 50 mg/L Tar can inhibit the proliferation of HUVECs, however nicotine has the tendency of stimulate the proliferation of HUVECs.关键词
焦油/尼古丁/增殖/脐静脉内皮细胞Key words
tar/nicotine/proliferation/umbilical vein endothelial cells分类
医药卫生引用本文复制引用
吴雨径,张晶,赖斌,李屹,魏玉杰,刘惠亮..不同浓度的烟焦油与尼古丁对脐静脉内皮细胞增殖作用的研究[J].湖南中医药大学学报,2016,36(6):24-28,5.基金项目
国家自然科学基金资助项目（81370315）。 （）
