海洋科学2016，Vol.40Issue(3)：1-9,9.DOI:10.11759/hykx20141113002
栉孔扇贝、虾夷扇贝及其杂交子代线粒体 COI 和 Cytb 基因遗传多样性分析
Genetic diversity analysis of Chlamys farreri, Patinopecten yes-soensis, and their offspring based on COI and Cytb sequences
摘要
Abstract
Mitochondrial DNA cytochrome oxidase subunit I (COI) and cytochrome b (Cytb) gene fragments were amplified and analyzed to examine the genetic diversity and structure of three scallop populations: Chlamys farreri, Patinopecten yessoensis, and their hybrid (F1). Fragments with 781 bp of the partial COI gene and 725 bp of the partial Cytb gene were obtained after alignment and emendation, and 47 haplotypes were found in the sequences of the 74 samples. The genetic diversity indexes, including the haplotype number, haplotype diversity, nucleotide diversity, and average number of nucleotide differences, showed that the genetic diversity of F1 was greater than that of the parents. The ge-netic distance between F1 and C. farreri was the nearest and that between F1 and P. yessoensis was the farthest. The level of gene flow between F1 and P. yessoensis was less than the level between F1 and C. farreri, while the population differentiation values between F1 and P. yessoensis was greater than the value between F1 and C. farreri, suggesting that the differentiation between C. farreri and P. yessoensis happened a long time ago. A UPGMA tree and reduced median network of haplotypes led to the same result, with the population of C. farreri and F1 gathered together and the population of P. yessoensis gathered together independently. PCR reamplification revealed that the special primers of C. farreri could be amplified but the P. yessoensis special primers could not be amplified in the F1 population, indi-cating that the mitochondrial DNA genetic model of F1 represented matrilinear inheritance.关键词
COI/Cytb/栉孔扇贝/虾夷扇贝/F1/遗传多样性Key words
COI/Cytb/Chlamys farreri/Patinopecten yessoensis/F1/genetic diversity分类
农业科技引用本文复制引用
于涛,吴彪,杨爱国,周丽青,刘志鸿..栉孔扇贝、虾夷扇贝及其杂交子代线粒体 COI 和 Cytb 基因遗传多样性分析[J].海洋科学,2016,40(3):1-9,9.基金项目
国家现代农业产业技术体系建设专项基金项目@@@@Supported by the earmarked fund for modern agroindustry technology research system （）
