海洋科学2016，Vol.40Issue(3)：10-16,7.DOI:10.11759/hykx20150826001
薄荷醇对仿刺参幼参的麻醉效果
Anesthetic effect of menthol on juvenile sea cucumber Apos-tichopus japonicus
摘要
Abstract
The aim of this study was to establish an anesthetic method for accurate body length measurement. We investigated the anesthetic effect of menthol on different-sized (large, 42.37 g±1.99 g; medium, 22.91 g±1.03 g;small, 12.09 g±1.51 g) Apostichopus japonicus juvenile sea cucumbers in different concentrations and at different water temperatures (11, 13, 15, 17, 19, 21℃). The results showed that the anesthetic effect of menthol took effect quickly and the recovery rate reached 100% at concentrations less than 4%. The anesthetic time and recovery time were significantly different for large individuals at concentrations of 0.25%, 0.5%, and 1%, but they were not sig-nificantly different for medium-sized and small ones. Within the temperature range from 11℃ to 19℃, the anes-thetic time for sea cucumbers was shortened from 15.62 min±1.31 min to 12.17 min±0.21 min, while the recovery time increased from 13.61 min±4.85 min to 28.10 min±7.35 min. The anesthetic effect was significantly different at different temperatures (P<0.05). It was unfit for anesthetization because the status of the sea cucumber was abnor-mal when the temperature was higher than 21℃. These results suggest that, within the temperature range from 11℃to 21℃, the effective anesthetic concentration of menthol is approximately 0.5% to 1% for individuals with body weight greater than 20 g and approximately 0.25% to 0.5% for individuals less than 20 g in body weight.关键词
仿刺参(Apostichopus japonicus)/薄荷醇/麻醉/温度Key words
Apostichopus japonicus/menthol/anesthetization/water temperature分类
农业科技引用本文复制引用
韩莎,胡炜,李成林,赵斌,严芳..薄荷醇对仿刺参幼参的麻醉效果[J].海洋科学,2016,40(3):10-16,7.基金项目
山东省农业良种工程项目(2014-2016) （2014-2016）
山东省科技发展计划项目(2014GNC111022) （2014GNC111022）
国家海洋局海洋公益性行业科研专项经费项目(201305001-4) （201305001-4）
山东省现代农业产业技术体系刺参产业创新团队建设项目(SDAIT-08-011-01) （SDAIT-08-011-01）
山东省农业科技成果转化资金项目@@@@Agriculture Seed Project of Shandong Province, No.2014-2016 （）
Science and Technology Development Project of Shan-dong Province, No.2014GNC111022 （）
Oceanic Research Special Funds for Public Welfare Projects of State Oceanic Administration, No.201305001-4 （）
Sea cucumber Innovation Team of Shandong Agriculture Research System, No.SDAIT-08-011-01 （）
Agricultural Science and Technology Achievement Transformation Project of Shandong Province （）
