海洋科学2016，Vol.40Issue(3)：40-46,7.DOI:10.11759//hykx20150305003
渗透压、pH、葡萄糖及离子溶液对夏牙鲆精子激活及运动特征的影响
Effects of osmolality, pH, glucose, and ion solutions on sperm motility of the summer flounder Paralichthys dentatus
摘要
Abstract
In the present study, we evaluated the effects of osmolality, pH, glucose, and ion solutions on the sperm motility of summer flounder (Paralichthys dentatus) using a computer-assisted sperm analysis system. According to the results, the moderate osmolality for sperm activation was 581–1260 mosm/kg under the osmolality ranges as follows: among group A (581–650 mosm/kg), B (762–877 mosm/kg), C (931–1060 mosm/kg), and D (1117–1180 mosm/kg). The glucose solution had an advantage over NaCl and KCl on sperm motility. Under high osmolality (1091–1180 mosm/kg), sperm motility significantly varied in EGTA400, CaCl2400, and artificial seawater without Ca2+. The optimum pH for the sperm activation of summer flounder was 7–7.5. In conclusion, the present study indicated that osmolality is the most pronounced factor influencing the sperm activation of summer flounder. Addi-tionally, glucose, exogenous Ca2+, and pH influence sperm motility to different degrees.关键词
夏牙鲆(Paralichthys dentatus)/精子/渗透压/pH/运动率Key words
Paralichthys dentatus/sperm/osmolality/pH/motility分类
生物科学引用本文复制引用
王学颖,徐世宏,刘清华,李军..渗透压、pH、葡萄糖及离子溶液对夏牙鲆精子激活及运动特征的影响[J].海洋科学,2016,40(3):40-46,7.基金项目
国家自然科学基金项目(31572602,31472264) （31572602,31472264）
鲆鲽类产业技术体系(nycytx-50) （nycytx-50）
海洋药源生物种质资源库建设(12PYY001SF08) （12PYY001SF08）
现代生态海洋农业科技创新(KSC2-EW-B-3) （KSC2-EW-B-3）
中国科学院 STS 项目(KFJ-EW-STS-060) （KFJ-EW-STS-060）
国家水产种质资源平台@@@@National Natural Science Foundation (No.31572602, No.31472264) （No.31572602, No.31472264）
Modern Agro-industry Technology Research System (No. nycytx-50) （No. nycytx-50）
Marine Economy Innovative Development Project(No.12PYY001SF08) （No.12PYY001SF08）
The Innovation Key Program of the Chinese Academy of Sciences (No. KSCX2-EW-B-3) （No. KSCX2-EW-B-3）
Chinese Academy of Science and Technology Service Network Planning (No. KFJ-EW-STS-060) （No. KFJ-EW-STS-060）
National Infrastructure of Fishery Germplasm Resource （）
