海洋科学2016，Vol.40Issue(3)：59-64,6.DOI:10.11759//hykx20150427001
饲料中添加两株乳酸菌及其发酵上清液对凡纳滨对虾消化酶活性的影响
Effects of two lactic acid bacteria species and the corre-sponding supernatants on the activities of digestive enzymes in Litopenaeus vannamei
摘要
Abstract
To investigate the effects of probiotics and the corresponding supernatants on the activities of digestive enzymes in Litopenaeus vannamei, several groups of L. vannamei (approximately 3.5 g) were reared at a tempera-ture of 30 ± 2°C in an aquaria for 4 weeks. The following four trials (three treated and one control) were conducted:T1, shrimp fed with a commercial diet + strain Lactobacillus pentosus HC-2 (1×107CFU/g feed); T2, shrimp fed with a commercial diet + strain Enterococcus faecium NRW-2 (1×107-CFU/g feed); T3, shrimp fed with a commer-cial diet + the supernatant of strain L. pentosus HC-2 (the corresponding supernatant of 1×107CFU/g feed of L. pentosus HC-2); and T4, shrimp fed with a commercial diet alone as a control. The results demonstrated that the protease activities were significantly (P<0.05) increased in the intestines of three treatment groups compared with those in the control group, but they were not significantly increased in the hepatopancreas. The amylase and lipase activities were significantly (P<0.05) increased in the intestines and hepatopancreas of shrimp fed with E. faecium NRW-2 compared with those in the control group. The amylase and lipase activities were significantly (P<0.05) increased in the hepatopancreas and intestines, respectively, in the group of shrimp fed with L. pentosus HC-2, whereas the protease and amylase activities were just increased significantly in the intestines of shrimp fed with the supernatant of L. pentosus HC-2. This study suggested that the activities of digestive enzymes in L. vannamei can increase by NRW-2, HC-2, and the corresponding supernatant but vary in different tissues. These results provide a solid foundation for the application of probiotics in aquaculture.关键词
戊糖乳杆菌(Lactobacillus pentosus)HC-2/粪肠球菌(Enterococcus faecium)NRW-2/凡纳滨对虾(Litopenaeus vannamei)/消化酶活性Key words
Lactobacillus pentosus HC-2/Enterococcus faecium NRW-2/Litopenaeus vannamei/digestive enzyme分类
农业科技引用本文复制引用
沙玉杰,王雷,孙国琼,刘梅,王宝杰..饲料中添加两株乳酸菌及其发酵上清液对凡纳滨对虾消化酶活性的影响[J].海洋科学,2016,40(3):59-64,6.基金项目
国家科技支撑计划(2012BAD17B03)@@@@The National Key Technology R&D Program of China, No.2012BAD17B03 （2012BAD17B03）
