激光生物学报2016，Vol.25Issue(2)：123-128,6.DOI:10.3969/j.issn.1007-7146.2016.02.005
紫铆因对食管鳞癌细胞增殖和存活影响的初步研究
A Study of the Effects of Butein on Esophageal Squamous Cell Carcinoma Cells Proliferation and Apoptosis
摘要
Abstract
To investigate the effects of butein on proliferation and apoptosis of human esophageal squamous cell carcino-ma cells.The proliferation of KYSE150 and Eca109 cells was tested by MTS and soft agar assay.Glycolysis was evalua-ted through measurement of glucose uptake and lactate production.Meanwhile,the signaling transduction pathway after butein treatment and the expression of the protein which was involved in proliferation and apoptosis were tested via immu-noblotting.We found that butein inhibited the proliferation of KYSE150 and Eca109 cells in a dose-dependent manner. The effects of butein mediated proliferation inhibition of human esophageal squamous cell carcinoma cells was related to the suppression of EGFR signaling pathway and down-regulation of glycolysis.A certain concentration of butein can di-rectly induce apoptosis in human esophageal squamous cell carcinoma cells,which result in the activation of caspase3/PARP and down-regulation of the expression of Bcl-2 and Mcl-1 .Our primary data demonstrated that the suppression ofEGFR signaling pathway and glycolysis,as well as down-regulation of Bcl-2 and Mcl-1 expression are involved in butein mediated proliferation inhibition and apoptosis in human esophageal squamous cell carcinoma.关键词
食管鳞癌/紫铆因/细胞增殖/糖酵解/凋亡Key words
esophageal squamous cell carcinoma/butein/cell proliferation/glycolysis/apoptosis分类
医药卫生引用本文复制引用
李伟,刘文斌,刘海丹..紫铆因对食管鳞癌细胞增殖和存活影响的初步研究[J].激光生物学报,2016,25(2):123-128,6.基金项目
国家自然科学基金 （）
