激光生物学报2016，Vol.25Issue(2)：129-135,7.DOI:10.3969/j.issn.1007-7146.2016.02.006
N rf2／HO-1／HMGB 1信号通路在白血病细胞 K562／A02化疗耐药中的机制研究
The Nrf2/HO-1/HMGB 1 Signaling Pathway is Associated with Chemotherapy Resistance in K562/A02 Leukemia Cells
摘要
Abstract
Objective:By silencing the high mobility group protein 1 (HMGB1 )gene,the nuclear factor erythroid 2-re-lated factor 2 (Nrf2)gene,and the heme oxygenase 1 (HO-1)gene in chemotherapy-resistant leukemia cells (K562/A02 cell line),to explore the role of the Nrf2/HO-1/HMGB1 signaling pathway in leukemia chemotherapy resistance and its possible mechanism.Methods:The Adriamycin-resistant cell line K562/A02 were transfected with specific small interfering RNAs (siRNA)of HMGB1 gene,Nrf2 gene,and and HO-1 gene respectively.Fluorescence quantitative re-al-time polymerase chain reaction was used to examine the mRNA expression levels of HMGB1,Nrf2,and HO-1.West-ern blot was used to determine the protein expression levels of HMGB1,Nrf2,and HO-1.The Nrf2 protein expression level was detected by immunofluorescence assay.Cell viability of K562/A02 cell line was analyzed by Cell Counting Kit-8 before and after transfection.Results:Silencing of HMGB1,Nrf2,or HO-1 in K562/A02 cell line enhanced cell via-bility and recovered chemosensitivity compared with the control group and the blank group (P<0.05 ).Conclusions:HMGB1 overexpression in K562/A02 cell line might lead to chemotherapy resistance to Adriamycin.The Nrf2/HO-1 signaling pathway might be involved in the HMGB1-induced chemotherapy resistance in K562/A02 cell line,and its up-regulation might recover chemosensitivity.关键词
白血病/化疗耐药/多药耐药/高迁移率族蛋白1/转录因子NF-E2相关因子2/血红素加氧酶1Key words
leukemia/chemotherapy resistance/multidrug resistance/high mobility group protein 1 (HMGB1 )/nu-clear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1)分类
医药卫生引用本文复制引用
赵明一,李嘉欣,陈志衡,高飞,赵平,戴云鹏,管国涛,刘丽英..N rf2／HO-1／HMGB 1信号通路在白血病细胞 K562／A02化疗耐药中的机制研究[J].激光生物学报,2016,25(2):129-135,7.基金项目
国家自然科学基金（81200378，81500231）；湖南省自然科学基金 （）
