激光生物学报2016，Vol.25Issue(2)：161-169,9.DOI:10.3969/j.issn.1007-7146.2016.02.011
鳜鱼消化道黏液细胞和6种酶的组织化学定位
Histochemical Localization of Mucous Cells and Six Types of Enzymes in Digestive Tract of Siniperca Chautsi
摘要
Abstract
The distribution and localization of mucous cells and six types of enzymes in the digestive tract of Siniperca chautsi were investigated by AB-PAS (alcian blue and periodic acid schiff’s reaction)staining and enzyme histochemi-cal method.The mucous cells could be divided into four types,most of mucous cells in esophagus were type Ⅲ and Ⅳcells,no typeⅠ and Ⅱ cells were found;only typeⅠmucous cells were detected in the mucosal epithelia of stomachus cardiacus and stomachus pyloricus,while the mucous cells in the stomachus corpus were mainly type Ⅲ cells;the mu-cous cells in pyloric caeca were mainly type Ⅱ cells;most of mucous cells in pre-intestine and mid-intestine were typeⅣ cells,typeⅠcells were the least ones;while the mucous cells in hind-intestine were mainly type Ⅱ and Ⅳ cells.Acid phosphatase(ACP)was mainly distributed in the mucosal epithelia of the pyloric caeca and pre-intestine;Alkaline phosphatase(ALP)mainly existed in the mucosal epithelia of the esophagus,pyloric caeca and the whole intestine;Nonspecific esterase (NSE)was mainly distributed in the mucosal epithelia of the stomachus pyloricus,mid-intestine and hind-intestine.Peroxidase(POX)was detected with higher activity in the mucosal epithelia of stomachus pyloricus. Succinate dehydrogenase(SDH)was mainly located in the gastric gland of stomach.Adenosine triphosphatase(ATPase) was detected with higher activity in the whole digestive tract.The distribution pattern of the mucous cells and enzymes in the digestive tract of Siniperca chautsi was similar to that of other animals,but some specific features have also been ob-served,which conform with the physiological function in the different parts of digestive tract.关键词
鳜鱼/消化道/黏液细胞/酶组织化学Key words
Siniperca chautsi/digestive tract/mucous cells/enzyme histochemistry分类
生物科学引用本文复制引用
彭迁迁,权梦雪,罗莹,孙德文,祝雪,张盛周..鳜鱼消化道黏液细胞和6种酶的组织化学定位[J].激光生物学报,2016,25(2):161-169,9.基金项目
安徽省高校省级自然科学研究重点项目（No．KJ2013A126）；国家级大学生创新创业训练计划项目（NO．201410370051）；生物环境与生态安全省级重点实验室及安徽省重要生物资源保护与利用重点实验室建设基金（2012-10）资助 （）
