临床口腔医学杂志2016，Vol.32Issue(6)：335-337,338,4.DOI:10.3969/j.issn.1003-1634.2016.06.006
氟离子植入猪骨源羟基磷灰石对破骨样细胞生物学行为影响的体外研究
Effects of fluoride incorporation into porcine hydroxyapatite on biological responses of osteoclast-like cells:An in vitro study
摘要
Abstract
Objective: To evaluate the effect of fluoridation of porcine hydroxyapatite on biological responses of osteo-clast-like cells. Method:Fluorinated porcine hydroxyapatite(FPHA) was fabricated from porcine bone by chemical immer-sion and thermal treatment. Osteoclast-like cells was induced from mouse monocyte-macrophage cell line RAW264.7. Osteo-clast-like cells attachment, differentiation and function was observed by scanning electron microscope. The viability of os-teoclast-like precursors was determined by cell counting kit-8. Tartrate resistant acid phosphatase (TRAP) activity was de-tected quantitatively by TRAP assay Kit. Result: Osteoclast-like cells could attach, differentiate and participate in materi-als resorption on PHA and FPHA disks. No significant inhibiting effect was observed in osteoclast-like precursors viability test. However, a decrease of TRAP activity was detected. Conclusion:FPHA could inhibit the bone resorption function of osteoclast-like cells.关键词
氟离子植入/猪骨源羟基磷灰石/破骨样细胞Key words
Fluoride incoperation/Porcine hydroxyapatite/Osteoclast-like cells分类
生物科学引用本文复制引用
罗欣,李志鹏,黄宝鑫,吴夏怡,胡铭轩,陈卓凡..氟离子植入猪骨源羟基磷灰石对破骨样细胞生物学行为影响的体外研究[J].临床口腔医学杂志,2016,32(6):335-337,338,4.基金项目
国家自然科学基金（81470783，81400550）广东省自然科学基金（2015A030311051，2016A030310173）广东省医学科研基金 （）
