山东农业大学学报（自然科学版）2016，Vol.47Issue(3)：345-352,8.DOI:10.3969/j.issn.1000-2324.2016.03.005
NaHCO3及培养时间对球等鞭金藻（Isochrysis galbana）生长、蛋白质和脂肪酸组成的影响
Effects of NaHCO3 and Culture Phase on Growth, Protein Content and Fatty Acid Composition of Isochrysis galbana
摘要
Abstract
The growth, cellular protein content and fatty acid profile in micro-algae Isochrysis galbana were valuated when the culture was supplemented with 0, 0.5, 1.0, 1.5 and 2.0 mg/mL NaHCO3 respectively. Then the protein content and fatty acid profile in cell at different growth phase were also investigated when the culture medium was added with 1.5 mg/mL NaHCO3. The results indicated there were significant effects of NaHCO3 supplementation on growth, cellular protein content and fatty acid profiles. The treatment supplemented with 1.5 mg/mL NaHCO3 displayed the highest cell density and special growth rate. The cellular protein content decreased significantly along with the increasing supplementation of NaHCO3 in culture medium. The monounsaturated fatty acids increased significantly while the polyunsaturated fatty acids decreased significantly along with the increase of NaHCO3 supplementation. There were significant differences in cellular protein content and fatty acid profile among the cells which were at a different growth phase when the cells were cultured in the medium supplemented with 1.5 mg/mL NaHCO3. The saturated fatty acids and monounsaturated fatty acids increased significantly while the polyunsaturated fatty acids decreased significantly along with the culture duration. Therefore it was suggested that NaHCO3 supplementation in culture medium was helpful for improving the micro-algae quality for bio-diesel production.关键词
球等鞭金藻/NaHCO3/生长/蛋白质/脂肪酸Key words
Isochrysis galbana/NaHCO3/growth/protein content/fatty acid分类
农业科技引用本文复制引用
王星宇,黄旭雄,杨碧莲..NaHCO3及培养时间对球等鞭金藻（Isochrysis galbana）生长、蛋白质和脂肪酸组成的影响[J].山东农业大学学报（自然科学版）,2016,47(3):345-352,8.基金项目
上海市科技兴农项目(沪农科推字(2013)第2-1号 （沪农科推字(2013）
沪农科攻字(2015)第1-2号) （2015）
