山东农业大学学报（自然科学版）2016，Vol.47Issue(3)：353-358,6.DOI:10.3969/j.issn.1000-2324.2016.03.006
30个马铃薯卷叶病毒CP基因序列分析
Analysis on CP Gene Sequences of Thirty Potato Leaf Roll Virus
摘要
Abstract
We used RT-PCR to amplify the different samples with PLRV and successfully cloned PLRV CP gene by means of comparing CP gene sequence of samples to discover 99.55%of the nucleic acid concordance rate, there was only five bases difference between the sample from Guangdong and others. We had analyzed the test samples and other recorded PLRV CP genes in China and divided them from different districts into Group A, Group B. The results showed that samples from Guangdong, Yunnan and Guizhou distributed in two groups but only the samples from Inner Mongolia cluster in Group A. The PLRV CP genes from Chinese samples compared with other countries to be divided into Group S1 and Group S2. In Group S1, there were unordered samples from China and most of other countries;in Group S2, there were only samples from Egypt and Poland. In one word, there were a little difference in PLRV CP sequences so that at present the gene of the PLRV population was stable.关键词
马铃薯/卷叶病毒/CP基因/序列分析Key words
Potato/potato leaf roll virus(PLRV)/CP gene/sequences analysis分类
农业科技引用本文复制引用
韩树鑫,张俊华,白艳菊,张威,高艳玲,范国权,张抒,申宇..30个马铃薯卷叶病毒CP基因序列分析[J].山东农业大学学报（自然科学版）,2016,47(3):353-358,6.基金项目
现代农业产业技术体系专项资金资助(CARS-10-P14) （CARS-10-P14）
