山西农业大学学报（自然科学版）2016，Vol.36Issue(5)：310-314,363,6.
山西西葫芦花叶病病原鉴定与部分序列分析
Identification and partial sequence analysis of the pathogen causing Cucurbita pepo L.mosaic disease in Shanxi province
摘要
Abstract
To identify the viruses that induced Cucurbita pepo L.mosaic disease,dsRNA technology and sequence-inde-pendent amplification (SIA)were used.Results of SIA and sequencing showed that Cucurbita pepo L.was infected by Cucumber mosaic virus (CMV).To further characterize the Shanxi isolate of Cucumber mosaic virus (CMV-XHL), the coat protein gene (CP )and movement protein gene (MP )coded by CMV-XHL RNA3 were cloned and sequenced. Sequence alignments showed that CMV-XHL has the highest identities of 98.6% in nucleotide level and 99.5% in de-duced amino acid level with the strain SG which belong to CMV subgroupⅠB and 93.2%~94.6% in CMV SubgroupⅠ.Phylogenetic analysis of CP and MP suggested that CMV-XHL belongs to CMV subgroupⅠ.The results of this study may serve as a theoretical basis to prevent and control Cucumber mosaic virus .关键词
西葫芦/黄瓜花叶病毒/病原鉴定/序列分析Key words
Cucurbita pepo L./Cucumber mosaic virus/Pathogen identification/Sequence analysis分类
农业科技引用本文复制引用
王德富,时晓丽,寇丽莎,庞小静,刘勇,牛颜冰..山西西葫芦花叶病病原鉴定与部分序列分析[J].山西农业大学学报（自然科学版）,2016,36(5):310-314,363,6.基金项目
公益性行业(农业)科研专项(No.201303028) （农业）
山西农业大学科技创新基金(20142-10) （20142-10）
山西农业大学引进人才科研启动基金(2014YJ05) （2014YJ05）
