山西农业大学学报（自然科学版）2016，Vol.36Issue(5)：315-319,5.
番茄APX基因的分离及表达分析
Isolation and expression analysis of LeAPX gene in tomato
摘要
Abstract
Imbalance of metabolism can cause increased accumulation of ROS.If it could not be scavenge timely,it will lead to damage to cellular components.In antioxidative system,Ascorbate peroxidase (APX)has been considered as pivotal enzyme for scanvenging ROS in chloroplasts and protecting cellular components.In the present study,we isola-ted an APX gene,designated LeAP X ,from tomato.The 1 412 bp cDNA contained an open reading frame (ORF)com-prising of 1 266 nucleotides (nt)which encodes a polypepetide of 421 amino acid residues.The deduced amino acid se-quence showed high identity with APXs from Solanum tuberosum ,Sesamum indicum and Nicotiana tabacum .Amino acid sequence alignment indicated that the APX contained two domains.Sequence analysis of the promoter indicates that it is rich in acting elements such as HSE、LTR、GT1. qPCR indicated that the gene showed the highest expression level in leaves comparing with other organs.关键词
番茄/抗坏血酸/APX基因Key words
Tomato/Ascorbate/AP X gene分类
生物科学引用本文复制引用
段明,王兴春,元旭朝..番茄APX基因的分离及表达分析[J].山西农业大学学报（自然科学版）,2016,36(5):315-319,5.基金项目
山西省青年科技研究基金(2015021142) （2015021142）
山西农业大学科技创新基金(20142-12) （20142-12）
