现代检验医学杂志2016，Vol.31Issue(3)：48-50,54,4.DOI:10.3969/j.issn.1671-7414.2016.03.013
禽流感病毒 H7N9型实时荧光定量RT-PCR检测方法的研究
Devolepment of Real-time Fluorescence Quantitative RT-PCR Detection for Avian Influenza Virus H7N9 Type
摘要
Abstract
Objective To establish a rapid and accurate method of fluorescent quantitative PCR detection for Avian Influenza Virus H7N9 type.Methods A pair of primers and TaqMan fluorescent probes were designed specificly for H7N9 gene ac-cording to the published nucleotide sequence from National Center for Biotechnology Information.Positive recombinant plas-mid was built and the minimum copies of detection,repeatability and specificitv were tested.Results The results showed that this assay obtained positive recombinant plasmid,the linear relation was fine in the range from 102 to 108 copies/μl.The minimum copies was 500 copies/μl with this method,specificitv and repeatability(CV<1%)were fine,no cross reaction with other viruses.The precision was 100% (16/16)with this method for detecting 8 positive samples and 8 negative sam-ples.Conclution The establishment methods of fluorescent quantitative PCR detection could be used for the rapid diagnosis Avian Influenza virus H7N9 types.关键词
禽流感病毒 H7N9 型/实时荧光定量/RT-PCR/检测Key words
avian influenza virus H7N9/real-time fluorescence quantitative/RT-PCR/detection分类
医药卫生引用本文复制引用
陈勉乔,于浩洋..禽流感病毒 H7N9型实时荧光定量RT-PCR检测方法的研究[J].现代检验医学杂志,2016,31(3):48-50,54,4.基金项目
深圳市技术创新计划（CYZZ20140605154742155）；深圳市南山技术研发和创意设计项目（KC2014JSJS0018A）。 （）
