现代检验医学杂志2016，Vol.31Issue(3)：98-101,4.DOI:10.3969/j.issn.1671-7414.2016.03.027
乙型肝炎病毒SYBR Green I实时荧光定量PCR方法的建立及临床应用
Establishment and Application of SYBR Green I Real-Time PCR Assay for Rapid Detection of Hepatitis B Virus DNA
摘要
Abstract
Objective To develop SYBR Green I real-time PCR assay for detection and identification of Hepatitis B virus. Methods Based on the sequences of Hepatitis B virus gp1 gene,primers were designed.The reaction assay and thermal cyc-ling profile were optimized.The positive standard was from recombinant clone.Both the developed assay and Zhejiang kuake biotechnology company’s assay were applied in 100 patients serum.Results The detection limit was between 5×102 copies/ml to 5×108 copies/ml with a good liner correlation and no cross reaction.The whole process just needed 2.5 h.Comparing with the company products,the sensitivity and specificity of the developed assay were 100% and 92.5% respectively.Con-clusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the identification of Hepatitis B virus patients,but also provide accurate quantitative analysis for HBV patients.关键词
SYBR Green I实时荧光定量PCR/乙型肝炎病毒DNA/快速检测/公司产品对比Key words
SYBR Green I real-time PCR/Hepatitis B virus DNA/rapid detection/company product comparation分类
医药卫生引用本文复制引用
何维娜,吕东月,刘和录,韩杰辉,何玥,李培培..乙型肝炎病毒SYBR Green I实时荧光定量PCR方法的建立及临床应用[J].现代检验医学杂志,2016,31(3):98-101,4.基金项目
深圳市宝安区科技计划项目（2014183）。 （）
