天津中医药2016，Vol.33Issue(6)：354-357,4.DOI:10.11656/j.issn.1672-1519.2016.06.10
天麻钩藤饮对自发性高血压大鼠心脏Bax及Caspase-3蛋白表达的影响
Effect of the Tianma Gouteng Yin regulating the protein expression of Bax and Caspase-3 of myocardial tissue in spontaneously hypertensive rats
摘要
Abstract
[Objective] To investigate the mechanism of Tianma Gouteng Yin (TMGTY) reducing blood pressure and the protein expression of Bax and Caspase-3 in myocardial tissue in spontaneously hypertensive rats. [Methods] Twenty 14-week-old SHR rats were randomly assigned to control group (n=6), TCM group (n=7) and Western medicine group (n=7). The rats in TCM group were given TMGTY with the dose of 20 g/(kg·d);those in the western medicine group were given ramipril [1 mg/(kg·d)];those in control group were given the same volume of pure water. Blood pressure and heart rate were measured weekly. After 4 weeks, abdominal aortic blood and sacrifice animals. Enzyme-linked immunosorbent assay was used to analyze the content of AngⅡand ALD in serum. Western blot was used to analyze quantitatively Bax and Caspase-3. [Results] 1) Starting from the third week after the administration, compared with the control group, TMGTY significantly reduced systolic blood pressure (P﹤0.05, P﹤0.01). Although not significant antihypertensive effect, TMGTY controlled the overall increase in systolic blood pressure, and systolic blood pressure gradually decreased. 2) TMGTY and ramipril had no significant effect on heart rate. 3) Compared with the control group, TMGTY and ramipril significantly reduced the content of AngⅡ and ALD in serum (P﹤0.01). 4) Compared with the control group, TMGTY and ramipril significantly reduced the protein expression of Bax and Caspase-3 in myocardial tissue (P﹤0.01). [Conclusion] TMGTY could effectively control the increase in systolic blood pressure in SHR. The antihypertensive mechanism of TMGTY may be reducing ALD and AngⅡ. Further, by reducing the protein expression of Bax and Caspase-3, TMGTY possibly inhibited the apoptosis of cardiomyocytes, and achieved protection to cardiomyocytes.关键词
天麻钩藤饮/降压/AngⅡ/ALD/Bax/Caspase-3Key words
Tianma Gouteng Yin/reducing blood pressure/AngⅡ/ALD/Bax/Caspase-3分类
医药卫生引用本文复制引用
时晓迟,柳威,王娟,赵英强..天麻钩藤饮对自发性高血压大鼠心脏Bax及Caspase-3蛋白表达的影响[J].天津中医药,2016,33(6):354-357,4.基金项目
国家自然科学基金面上项目（81273941/H2902天津市自然科学基金资助项目（9JCZDJC20600）。 （）
