天津中医药2016，Vol.33Issue(6)：358-362,5.DOI:10.11656/j.issn.1672-1519.2016.06.11
消岩汤对A549/DDP细胞自噬及耐药蛋白的影响研究
Study on the autophagy and resistance protein affected by Xiaoyan decoction in A549/DDP cells
摘要
Abstract
[Objective] Through the cisplatin resistance of the human lung adenocarcinoma cells A549/DDP multi-resistant and influ-ence of autophagy protein by Xiaoyan decoction, finding out the influence of elimination of cisplatin resistance of lung adenocarcinoma A549/DDP resistance reversal agents and explore relevant molecular pathways. [Methods] Establish a stable cell resistance model, con-tinuous lavaging Xiaoyan decoction with different concentrations in 10 days, by serum pharmacology experiment method with drug-con-taining serum on lung adenocarcinoma cells resistant cells A549/DDP, through the QRT-PCR and Werstern Blot western Blot method to detect A549/DDP resistant, autophagy protein expression of related genes and related pathways. [Results] Resistance effect on lung ade-nocarcinoma cells A549/DDP with high dose Xiaoyan decoction, low dose Xiaoyan Decoction and normal saline, elimination saline resis-tance effect on lung adenocarcinoma cells A549/DDP, detecting drug-resistant related proteins in A549/DDP cell P-gp, LRP and Beclin1 protein involved in autophagy, HMGB1mRNA and protein expression changes,through the detection of A549/DDP cells drug-resistant related proteins P-gp, LRP and Beclin1 protein involved in autophagy, HMGB1mRNA and protein expression changes, high dose group of rock soup elimination processing cell lines after P-gp, LRP and Beclin1 protein involved in autophagy, both the HMGB1 mRNA and pro-tein were lower (P﹤0.05). Further found that Xiaoyan decoction can affect the AKT1-elimination mTOR related genes and significantly re-duce the AKT1, mTOR protein expression. [Conclusion] We hypothesized that Xiaoyan decoction influence elimination drug-resistant related proteins P-gp, LRP and Beclin1 protein involved in autophagy, HMGB1 genetic changes by AKT1-mTOR molecular pathways.关键词
消岩汤/细胞自噬/耐药/分子通路Key words
Xiaoyan decoction/autophagy/resistance/molecular pathway分类
医药卫生引用本文复制引用
杨佩颖,许文婷,刘宏根,张欣,张莹,于晓宇,贾英杰..消岩汤对A549/DDP细胞自噬及耐药蛋白的影响研究[J].天津中医药,2016,33(6):358-362,5.基金项目
国家自然科学基金资助项目（81273937），天津市科委自然科学基金青年项目（13JCQNJC10900），天津市抗癌重大专项攻关计划（12ZCDZSY15800）。 （）
