天津中医药2016，Vol.33Issue(6)：368-372,5.DOI:10.11656/j.issn.1672-1519.2016.06.13
蛇床子香豆素类成分的含量测定及指纹图谱研究
Simultaneous determination of coumarins and fingerprint analysis in Cnidium monnieri (L.) Cuss
摘要
Abstract
[Objective] A fingerprint analysis and simultaneous determination of six coumarins were established and validated to evaluate the quality of different batches of Cnidium monnieri (L.) Cuss. [Methods] The contents of xanthotoxin, isopimpinelline, bergapten, imperatorin, osthole and isoimperatorin were determined by HPLC. The analysis was carried out on Hedra ODS-2 column (4.6 mm × 250 mm, 5μm). The mobile phase was methanol-0.1%formic acid water aqueous solution in gradient elution. The flow rate was 0.5 mL/min and the detection wave length was 320 nm. [Results] The contents of six components in various bathes of samples were determined and HPLC fingerprint was established. The similarities of 17 batches of Cnidium monnieri (L.) Cuss. were more than 0.980. [Conclusion] The established method could be suitable to evaluate the quality control of Cnidium monnieri (L.) Cuss.关键词
高效液相色谱法/蛇床子/指纹图谱/香豆素类化合物Key words
HPLC/Cnidium monnieri (L.) Cuss./fingerprint/coumarins分类
医药卫生引用本文复制引用
宋丽娇,李晋,姜艳,陈莹,马琳,金华,常艳旭..蛇床子香豆素类成分的含量测定及指纹图谱研究[J].天津中医药,2016,33(6):368-372,5.基金项目
国家自然科学基金青年项目（81503213）；天津市高等学校创新团队培养计划项目（TD12-5033）。 （）
