现代医药卫生2016，Vol.32Issue(11)：1618-1621,4.DOI:10.3969/j.issn.1009-5519.2016.11.006
LIGHT作为EBNA1 DNA疫苗佐剂的免疫效应研究
Study on immune effect of LIGHT as adjuvant of EBNA1 DNA vaccine
卢晓华 1邹军辉 2郑威 3杨建设 3王蒲1
作者信息
- 1. 西北师范大学生命科学学院，甘肃 兰州730070
- 2. 中国科学院深圳先进技术研究院生物医药与技术研究所，广东深圳518055
- 3. 中国科学院深圳先进技术研究院生物医药与技术研究所，广东深圳518055
- 折叠
摘要
Abstract
Objective To study the effect of LIGHT as an adjuvant in the immune responses induced by EB viral nuclear antigen1(EBNA1) DNA vaccine. Methods The liver cDNA of Balbc/c mouse served as the template. The eukaryotic expression vector pcDDNA3.1-LIGHT was constructed by PCR amplification;the cell transfection was conducted,the LIGHT protein expres-sion was verified;by adopting the electric pulse method,the mouse was immunized with the LIGHT plasmid as the adjuvant com-bined with EBV DNA vaccine. The specific antibody of serum EBNA1 was detected. The mouse spleen lymphocytes were separat-ed and collected. After stimulation by EBNA1 antigen ,cytokine IFN-γsecretion level was detected. Results The LIGHT target fragment was amplified by PCR,which was about 720 bp,the Western blot identification results showed that a single clear target band was above 26 ×103,demonstrating that LIGHT protein was expressed;the serum detection results in the immune mouse showed that the antibody titer in the LIGHT combined DNA vaccine experimental group was 3 times of that in the single DNA vac-cine immune group;the spleen lymphocyte factor detection results showed that the LIGHT combined immune group could signifi-cantly increase the IFN-γsecretion. Conclusion LIGHT as the adjuvant has significant promoting effect in the generation of an-tibody and secretion of related cytokines by depending on its mediated signal pathway.关键词
疱疹病毒4型,人/疫苗,DNA/佐剂,免疫/LIGHT/电脉冲免疫Key words
Herpesvirus 4,human/Vaccines,DNA/Adjuvants,immunologic/LIGHT/Electroporation引用本文复制引用
卢晓华,邹军辉,郑威,杨建设,王蒲..LIGHT作为EBNA1 DNA疫苗佐剂的免疫效应研究[J].现代医药卫生,2016,32(11):1618-1621,4.
