湖北工程学院学报2016，Vol.36Issue(3)：16-21,6.
喹啉双季铵盐与小牛胸腺DNA的作用研究
Study on Interaction of Quinolinebiquaternary Ammonium Salt with Calf Thymus DNA
田瑜 1刘汉明 2陈静 1金义杰 1夏彩芬1
作者信息
- 1. 湖北工程学院 化学与材料科学学院，湖北 孝感 432000
- 2. 湖北省孝昌县第一中学，湖北 孝昌 432900
- 折叠
摘要
Abstract
The interaction between quinolinebiquaternary ammonium salt (BZQN)and calf thymus de-oxyribonucleic (ctDNA)was investigated by spectroscopy and isothermal titration calorimetry (ITC). The results indicated that BZQN had significant fluorescence.In the presence of ctDNA,the fluores-cence of BZQN was quenched and the quenching constant(Ksv )increased as the temperature rose, which fitted the characteristics of dynamic fluorescence quenching.All of the UV spectra,melting ex-periment and CD spectra showed that the conformation of ctDNA secondary structure had changed be-cause of the intercalation effect between BZQN and DNA.The ITC calculated that the binding point between BZQN and DNA was 4,and the combination process of BZQN and DNA was driven by entropy.关键词
喹啉双季铵盐/小牛胸腺DNA/光谱法/等温滴定微量热Key words
quinolinebiquaternary ammonium salt(BZQN)/calf thymus DNA(ctDNA)/spectrosco-py/isothermal titration calorimetry(ITC)分类
农业科技引用本文复制引用
田瑜,刘汉明,陈静,金义杰,夏彩芬..喹啉双季铵盐与小牛胸腺DNA的作用研究[J].湖北工程学院学报,2016,36(3):16-21,6.
