新医学2016，Vol.47Issue(6)：357-361,5.DOI:10.3969/j.issn.0253-9802.2016.06.003
纳米金颗粒对大鼠血脑屏障结构的影响
Effect of Au nanoparticles on the blood-brain barrier of rats
摘要
Abstract
Objective To study the effect on the structure of blood-brain barrier after the intravenous administration of Au nanoparticles to the rats.Methods 50 nm Au nanoparticles were intravenous administra-ted into rats through jugular vein.CSF was then collected with a cauda equine catheterization technique at 1 , 2,3,4,5 and 6 hours respectively after the administration.And the concentration of Au was detected by ICP-MS analysis.After injection the nanoparticles of 6 hours,the brains of the rats were collected and stored as fixed films,and then to observe the nanoparticles and ultrastructure of brain tissue under the TEM.Other sam-ples were stained by Prussian blue dye,Immunohistochemistry and Immunofluorescence and observed bleeding and integrity of the blood-brain barrier under microscope after making slices of brain tissue in rats.Results The concentration of Au nanoparticles in CSF gradually increased and stabilized after 3 hours.Under TEM,a large amount of Au nanoparticles appeared in the brain tissue and there were some changes of the ultrastructure of the brain tissue.Under microscope,there were hemorrhages after blood-brain barrier in rats were stained by Prussian blue-positive dye and some brain blood vessels were damaged in the Immunofluorescence and Immuno-histochemistry staining slice.Conclusion Au nanoparticles are able to permeate the brain-blood barrier and cause micro bleedings and micro damages of brain vessel.关键词
纳米颗粒/血脑屏障/大鼠/透射电镜Key words
Nanoparticles/Blood-brain barrier/Rat/Transmission electron引用本文复制引用
黄昱昊,何敏彤,杨圳冲,廖智恒,许衍扬,李梦圆,柳嘉欣,顾怀宇..纳米金颗粒对大鼠血脑屏障结构的影响[J].新医学,2016,47(6):357-361,5.基金项目
广东省科技计划项目 （）
