新医学2016，Vol.47Issue(6)：380-383,4.DOI:10.3969/j.issn.0253-9802.2016.06.008
外周血循环肿瘤 DNA 基因突变检测在非小细胞肺癌中的应用价值
The value of detection of gene mutations in peripheral circulating tumor DNA in non-small cell lung cancer
摘要
Abstract
Objectives To investigate the value of gene mutations in peripheral circulating tumor DNA (ctDNA)in non-small cell lung cancer (NSCLC)by next-generation sequencing.Methods The gene mutations in peripheral ctDNA and tDNA of tissued paraffin sections in 32 advanced patients with NSCLC were simultaneously detected by next-generation sequencing,and regarded the tissue tumor DNA as the correct standard to evaluate the efficiency of ctDNA diagnosing the gene mutations in NSCLC.Results Among 32 pa-tients with NSCLC,42 samples of gene mutations in 9 types were detected in terms of ctDNA and 40 samples of gene mutations in 1 1 types were detected in tDNA.The result of tDNA gene mutations was regarded as the cor-rect standard.Sensitivity of detection of gene mutations in peripheral circulating tumor DNA was 75%~1 00%, and specificity was 95%~1 00%.The concordance rate between result of tDNA and ctDNA was 91 %~1 00%. Conclusion To test mutations in NSCLC patients,detection of ctDNA is possible to replace tumor tissue par-affin sections by NGS.关键词
非小细胞肺癌/高通量测序/循环肿瘤脱氧核糖核酸Key words
Non-small cell lung cancer/Next-generation sequencing/Circulating tumor DNA引用本文复制引用
徐敏,何婉,李岚,朱美琴,陈亦欣,许瑞莲..外周血循环肿瘤 DNA 基因突变检测在非小细胞肺癌中的应用价值[J].新医学,2016,47(6):380-383,4.基金项目
深圳市科技计划项目 （）
