局解手术学杂志2016，Vol.25Issue(5)：323-326,4.DOI:10.11659/jjssx.01E016087
IFIT1在放射损伤与感染应激中表达的特点和意义
Characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radia-tion injury and infection stress
摘要
Abstract
Objective To explore characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress.Methods RNA was extracted from Raw264.7 cell,3T3 cell and 10T1 /2 cell after 5 hours stimulated with 5 μg/mL LPS.At the same time,to set up normal control group (untreated by LPS),and RNA of IFIT1 was detected by RT-PCR.Total-ly 20 C57 /BL6 mice were randomly divided into 4 groups,namely 0 h group,1 h group,4 h group and 12 h group.The mice were given 12 Gray60Co full-body exposure once,then liver IFIT1 was detected by western blot.Results Stimulated with LPS for 5 hours,IFIT1 was in-duced expression in Raw264.7 cell,3T3 cell and 10T1 /2 cell.The expression of normal control group was negative.The level of IFIT1 /Actin increased significantly 1 hour after radiation injury,and it reached the peak 12 hours after radiation injury (P <0.01).Conclusion LPS can stimulated a variety of cell lines expressed IFIT1,prompting that IFIT1 may participate in the occurrence and development of post-traumatic toxemia.IFIT1 of liver tissue increased significantly during the early stage in radiation mice.关键词
放射性损伤/脂多糖/IFIT1/糖皮质激素受体/毒血症Key words
radiation injury/lipopolysaccharide/IFIT1/glucocorticoid receptors/toxemia分类
医药卫生引用本文复制引用
郭晓姝,郝玉徽,粟永萍,王军平,王崧,许杨..IFIT1在放射损伤与感染应激中表达的特点和意义[J].局解手术学杂志,2016,25(5):323-326,4.基金项目
“十二五”国家科技支撑计划项目（2013BAK03B05-02）；国家重点实验室开放课题（SKLKF201321）；国家重点实验室自主研究课题 （）
