中国康复理论与实践2016，Vol.22Issue(6)：629-634,6.DOI:10.3969/j.issn.1006-9771.2016.06.002
低氧后处理对全脑缺血大鼠认知功能及海马CA1区沉默信息调节因子1表达的影响
Effects of Hypoxic Post-conditioning on Cognitive Function and Expression of Silent Information Regulator 1 in Hippocampal CA1 of Rats with Global Cerebral Ischemia
摘要
Abstract
Objective To explore the effects of hypoxic post-conditioning on cognitive function and the expression of silent information regulator 1 (SIRT1) in hippocampal CA1 of rats with cerebral ischemia. Methods Sixty Sprague-Dawley rats were randomly divided into sham operation group, model group and treatment group with 20 cases in each group. Each group was divided into one day, two days, three days, seven days subgroups according to the time of ischemia reperfusion. Global cerebral ischemia reperfusion was induced with modified Pulsinelli´4-vessel occlusion. The treatment group received 8%oxygen for two hours after ischemia. The cognitive function was assessed with Morris water maze test. Morphological changes of the hippocampal CA1 region were observed by HE staining. The expression of SIRT1 in the hippocampal CA1 region was detected with immunohistochemical assay and Western blotting. Results Compared with the model group, the escape latency significantly shortened (P<0.05), the number of times crossing the platform increased (P<0.05), the speed and the percentage of time spent in the platform quadrant increased (P<0.05), and the total distance decreased (P<0.05);the expression of SIRT1 in hippocampal CA1 increased (P<0.05) and the number of normal neurons increased (P<0.05) in the treatment group. Conclusion Hypoxic post-conditioning can improve the cognitive function of rats with global cerebral ischemia, which may relate with up-regulating SIRT1 in hippocampus.关键词
脑缺血/低氧后处理/认知功能/沉默信息调节因子1/大鼠Key words
cerebral ischemia/hypoxic post-conditioning/cognitive function/silent information regulator 1/rats分类
医药卫生引用本文复制引用
刁莉君,李建民,赵雅宁,陈长香,周娜..低氧后处理对全脑缺血大鼠认知功能及海马CA1区沉默信息调节因子1表达的影响[J].中国康复理论与实践,2016,22(6):629-634,6.基金项目
1.河北省自然科学基金资助项目(No. ZD2013093)；2.河北省自然科学基金资助项目(No. H2012401007)。 （No. ZD2013093）
