中国康复理论与实践2016，Vol.22Issue(6)：655-659,5.DOI:10.3969/j.issn.1006-9771.2016.06.007
Ser473-Akt磷酸化介导阿托伐他汀保护大鼠脑缺血再灌注损伤
Protective Effect of Ser473-Akt Phosphorylation Mediated Atorvastatin on Cerebral Ischemia-reperfusion Inju-ry in Rats
摘要
Abstract
Objective To investigate the effect of Ser473-Akt phosphorylation in the protection of atorvastatin to cerebral ischemia-re-perfusion (I/R) injury in rats. Methods Forty male Sprague-Dawley rats were randomly divided into normal group (n=10), sham group (n=10), I/R group (n=10) and intervention group (n=10). A model of cerebral ischemia-reperfusion in rats was establishied, with ischemia for 2 hours and reperfusion for 72 hours. The normal group and the sham group received no injury. I/R group was administered with normal saline only, and the intervention group received atorvastatin 10 mg/kg prepared with normal saline at palinesthesia, 24 and 48 hours after reperfu-sion. All rats were sacrificed 72 hours after reperfusion. HE staining and TUNEL staining were performed in the brain specimens. The ex-pression of Akt and Ser473-Akt in the prefrontal cortex of the brain were detected with Western blotting. Results Compared with I/R group, 72 hours after reperfusion, the damage of nerve cells significantly lessened in the intervention group;the apoptosis positive cells significant-ly reduced in the intervention group (t=-6.014, P<0.001). The expression of Ser473-Akt in prefrontal cortex was higher in I/R group than in the normal group and the sham group (t>20.327, P<0.001), and was higher in the intervention group than in I/R group (t=3.649, P=0.007). Conclusion The Ser473-Akt phosphorylation plays an important role in the protection of atorvastatin in nerve cell through anti-apoptosis of nerve cells, and reducing cerebral I/R injury.关键词
脑缺血/再灌注/Ser473-Akt/磷酸化/阿托伐他汀/细胞凋亡/大鼠Key words
cerebral ischemia-reperfusion/Ser473-Akt/phosphorylation/atorvastatin/apoptosis/rats分类
医药卫生引用本文复制引用
陶希,卢伟,胡治平,宋涛,邓景贵,蔡华安,王淑玲,刘佳..Ser473-Akt磷酸化介导阿托伐他汀保护大鼠脑缺血再灌注损伤[J].中国康复理论与实践,2016,22(6):655-659,5.基金项目
湖南省医药卫生科研计划项目(No. B2010-090)。 （No. B2010-090）
