农学学报2016，Vol.6Issue(5)：69-72,4.
中性条件下2种酶解方法提取蚕蛹蛋白的比较研究
Two Enzymatic Hydrolysis Methods for Extraction of Silkworm Pupa Protein Under Neutral Condition
摘要
Abstract
To explore an efficient and economic method for enzymatic hydrolysis of silkworm pupa protein, using fresh pupa and dried pupa as raw materials, the effect of neutral protease and flavourzyme on the degree of hydrolysis and protein yield was examined under neutral condition. The results showed that fresh pupa could obtain higher protein yield and higher degree of hydrolysis than dried pupa. The protein yield of enzymatic hydrolysis using flavourzyme was higher than that of neutral protease, but its hydrolysis degree was lower. This might be caused by the low endonuclease activity of flavourzyme. So the fresh pupas were preferred in practice and proper protease should be used according to different requirements of protein hydrolysate.关键词
蚕蛹蛋白/酶解/蛋白收率/水解度Key words
Silkworm Pupa Protein/Enzymatic Hydrolysis/Protein Yield/Degree of Hydrolysis分类
农业科技引用本文复制引用
张丽丽,章玉萍,陈明,代君君,吴传华,范涛..中性条件下2种酶解方法提取蚕蛹蛋白的比较研究[J].农学学报,2016,6(5):69-72,4.基金项目
安徽省农业科学院院长青年创新基金项目“蚕蛹蛋白生物活性肽抗氧化机理的研究”(15B0626)、“家蚕彩色茧中色素和丝心蛋白结合的反应机理研究”(13B0631)；安徽省农业科学院学科建设项目“蚕蛹蛋白的加工利用及其生理功效研究”(14A0607)；安徽省农业科学院科技创新团队项目“经济昆虫资源多功能利用创新与应用”(15C0606)；安徽省蚕桑产业技术体系专项(ahnycytx-16)；安徽省农业科学院博士科研启动基金项目“桑椹花色苷的提取纯化及对紫外线诱导氧化损伤机制的初步研究”；安徽省农业科学院种子工程项目“经济昆虫的种质资源保育与开发利用”(14D0606)。 （15B0626）
