中国药理学通报2016，Vol.32Issue(6)：773-778,6.DOI:10.3969/j.issn.1001-1978.2016.06.008
天然活性分子isatin经p53介导的线粒体途径诱导乳腺癌细胞MCF-7凋亡
The endogenous oxindole isatin induces apoptosis of MCF-7 breast cancer cells through a p53-mediated mitochondrial pathway
摘要
Abstract
Aim Toinvestigatetheinducementeffect of isatin on apoptosis of breast cancer cell line MCF-7 , andexploreitsdetailedmechanism.Methods MCF-7 cell lines were exposed to isatin at different concentra-tions(0,50,100,200 μmol·L-1 )for 48 h.Apop-totic features were demonstrated by nuclei staining with Hoechst 33258.Bcl-2,Bax and p53 mRNA were ana-lyzed by RT-PCR.Caspase-9 activation and mitochon-drial depolarization were assayed by flow cytometry. Bcl-2,Bax,p53 and cytochrome c proteins were ana-lyzedbyWesternblot.Results Isatininducesapopto-sis of MCF-7 cells.Furthermore,Bcl-2 expression was decreased and the ratio of Bcl-2 to Bax was significant-ly decreased by isatin.While,p53 expression relative-ly decreased.The mitochondrial transmembrane poten-tial was markedly reduced and the release of cyto-chrome c into the cytosol was increased after treatment with isatin.Simultaneously,caspase-9 was activated. Conclusions Isatinsignificantlyinducedtheapopto-sis of MCF-7 cells in vitro.These results strongly sug-gest that the p53 dependent mitochondrial pathway is involved in apoptosis.关键词
吲哚醌/乳腺癌细胞/凋亡/p53/Bcl-2/Bax/线粒体膜电位Key words
isatin/human breast cancer cell/apopto-sis/p53/Bcl-2/Bax/mitochondrial membrane poten-tial分类
医药卫生引用本文复制引用
宋金莲,马中良,迟晓伟,陈艳萍,侯琳..天然活性分子isatin经p53介导的线粒体途径诱导乳腺癌细胞MCF-7凋亡[J].中国药理学通报,2016,32(6):773-778,6.基金项目
国家自然科学基金项目（No 81472542）；青岛市卫生局项目（No 2014WJZD135）；青岛市科技局项目 （）
