中国药理学通报2016，Vol.32Issue(6)：779-782,783,5.DOI:10.3969/j.issn.1001-1978.2016.06.009
豆蔻明对TLR4／MyD88／NF-κB／iNOS信号通路的调节作用
The regulatory effect of cardamonin on TLR4/MyD88/NF-κB/iNOS pathway
摘要
Abstract
Aim Toassesstheregulatoryeffectsofcar-damonin (CDN ) on toll-like receptor (TLR )-4/MyD88/NF-κB/iNOS signaling pathway in lipopolysac-charide (LPS )-stimulated RAW264. 7 macrophage cells.Methods LPS-stimulatedRAW264.7cells were divided into three groups:vehicle-treated group, LPS-treated group and LPS +CDN-treated group.Cell viability was assessed by CCK-8 assay.The concentra-tion of nitric oxide (NO)in cell culture medium was measured by Griess reagent.The mRNA levels of iN-OS,COX-2,MCP-1 ,TNF-α,IL-6 and IL-1βwere de-termined by reverse transcription real-time quantitative PCR(RT-qPCR).The protein levels of inducible nitric oxide synthase(iNOS),TLR4,myeloid differentiation factor 88(MyD88),nuclear factor κB(NF-κB)phos-phorylated (p )-p65 ,inhibitor κBα(IκBα),and p-IκBαweredeterminedbyWesternblot.Results 1~50 μmol·L-1 CDN had no cytotoxicity in RAW264. 7 cells.However,CDN inhibited the LPS-induced secre-tion of nitric oxide(NO)and mRNA expressions of iN-OS,COX-2,MCP-1 ,TNF-α,IL-6 and IL-1βin a dose-dependent manner.Moreover,50 μmol · L-1 CDN inhibited the LPS-induced up-regulation of iNOS, TLR4,MyD88,NF-κB p-p65,p-IκBαand down-reg-ulationofIκBα.Conclusion Cardamonininhibitsthe production of NO via a mechanism associated with the inhibition of TLR4/MyD88/NF-κB/iNOS pathway.关键词
RAW264.7细胞/TLR4/MyD88/NF-κB/iNOS/豆蔻明Key words
RAW264.7 cell/TLR4/MyD88/NF-κB/iNOS/cardamonin分类
医药卫生引用本文复制引用
邓超,任改艳,孙阿宁,罗晓平,王峥涛,窦薇..豆蔻明对TLR4／MyD88／NF-κB／iNOS信号通路的调节作用[J].中国药理学通报,2016,32(6):779-782,783,5.基金项目
国家自然科学基金资助项目（No 81273572）；上海市高校一流学科创新研究基金资助项目（No ZYX-CXYJ-023）． （）
