中国药理学通报2016，Vol.32Issue(6)：783-788,6.DOI:10.3969/j.issn.1001-1978.2016.06.010
苯环喹溴铵对脂多糖诱导的人鼻黏膜上皮细胞表达MUC5 AC的影响
Effects of bencycloquidium bromide on the expression of MUC5 AC induced by lipopolysaccharide in cultured human nasal epithelial cells
摘要
Abstract
Aim Toinvestigatetheeffectofbencyclo-quidium bromide(BCQB)on mucus MUC5AC expres-sion induced by lipopolysaccharide in cultured human nasalepithelialcells(HNECs).Methods Primary culture of human nasal epithelial cells (HNECs)was randomly divided into control group (C,with no treat-ment),LPS group (LPS,with LPS 1 mg · L-1 added in),BCQB low dose group(BCQBL,with LPS 1 mg· L-1 and BCQB 10 -8 mol·L-1 added in),BCQB mid-dle dose group(BCQBM,with LPS 1 mg·L-1 and BC-QB 10 -7 mol·L-1 added in),BCQB high dose group (BCQBH,with LPS 1 mg·L-1 and BCQB 10 -6 mol· L-1 added in)and ipratropium bromide group(IB,with LPS 1 mg·L-1 and IB 10 -6 mol·L-1 added in).Af-ter incubation at 37 ℃with 5% CO2 for 24 h,the ex-pression of MUC5 AC mRNA was detected with Real-time PCR and the expression of MUC5 AC protein in HNECs was detected with Western blot,while the ex-pression of MUC5 AC protein in supernatant was detec-tedwithELISAineachgroup.Results Ascompared with control group,the expression of MUC5 AC mRNA and protein increased significantly in LPS group (each P<0. 01 ).As compared with LPS group,the expres-sion of MUC5 AC mRNA and protein decreased signifi-cantly in each group of BCQB(P<0. 01,P<0. 05), and there was no statistical difference between BCQB high dose group and control group (each P>0. 05 ). Conclusion Bencycloquidiumbromidecansuppress MUC5 AC expression induced by LPS in cultured hu-man nasal epithelial cells,indicating that BCQB may be a new drug for nasal mucous hypersecretion diseases.关键词
苯环喹溴铵/脂多糖/鼻黏膜/上皮细胞/黏液/MUC5 ACKey words
bencycloquidium bromide/lipopolysaccha-ride/nasal mucosa/epithelial cell/mucus/MUC5 AC分类
医药卫生引用本文复制引用
杨敏,陆雪,黄江菊,龙锐,李娟..苯环喹溴铵对脂多糖诱导的人鼻黏膜上皮细胞表达MUC5 AC的影响[J].中国药理学通报,2016,32(6):783-788,6.基金项目
国家自然科学基金青年基金资助项目（No 81200008）；国家临床重点专科项目（No 卫办医政函［2012］649号）；重庆市卫计委科研项目 （）
