中国药理学通报2016，Vol.32Issue(6)：789-794,6.DOI:10.3969/j.issn.1001-1978.2016.06.011
纳米颗粒对人食管癌EC-9706、EC-109细胞增殖和凋亡的影响
Effects of three kinds of nanoparticles on proliferation and apoptosis of esophageal squamous carcinoma cells
摘要
Abstract
Aim TostudytheeffectsofCuO,ZnOand TiO2 nanoparticles on the viability and metastatic po-tential of EC-9706 and EC-109 esophageal squamous carcinomacelllineinvitro.Methods Characteristics of CuO,ZnO and TiO2 nanoparticles were detected u-sing transmission electron microscope (TEM)and dy-namic light scattering (DLS ).EC-9706 and EC-109 cells were treated with different concentrations of CuO, ZnO and TiO2 (5 ~80 mg · L-1 ).The cell prolifera-tion was analyzed by MTT assay.The cell cycle and apoptotic rates were determined by flow cytometry (FCM).The cell invasion was assayed in Transwell chambers.The expression of Bcl-2 and caspase-3 pro-tein in cells was detected by Western blot method.Re-sults CuO,ZnOandTiO2nanoparticleswerespheri-cal with primary particle size 12,20. 6,12 nm.The particles were agglomerated in water and cell culture medium with negative charge.CuO and ZnO nanoparti-cles induced decreases in EC-9706 and EC-109 cell vi-ability dose-dependently.After exposed to increasing concentrations of CuO and ZnO nanoparticles,the cell cycle analysis revealed a decreasing proportion of cells in G2/Mand S phase,and up-regulation of the cells in G0/G1 phase.Apoptotic cells also increased along with decreased cell invasion upon CuO and ZnO treatment. Nanoparticles treatment after 48 h, the activated caspase-3 expression quantity increased significantly and the Bcl-2 expression quantity decreased obviously (P<0. 05 )compared with control group.TiO2 nanop-articles had no obvious effect on the EC-9706 and EC-109 cell proliferation,cell cycle,apoptosis and inva-sion.Conclusion ComparedwithTiO2,CuOand ZnO nanoparticles can inhibit EC-9706 and EC-109 cell viability and metastatic potential,the mechanism of action involves cell cycle arrest in G0/G1 phase and apoptosis.These findings can help the development of nanoparticles as anti-cancer therapeutics for esophageal cancer.关键词
食管癌/氧化铜纳米颗粒/氧化锌纳米颗粒/二氧化钛纳米颗粒/细胞增殖/凋亡Key words
esophageal cancer/CuO nano/ZnO nano/TiO2 nano/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
韩鹏黎,孙蕾,吕朋举,龚芬芬,马超,陈国,朱怡然,夏天,曹巍..纳米颗粒对人食管癌EC-9706、EC-109细胞增殖和凋亡的影响[J].中国药理学通报,2016,32(6):789-794,6.基金项目
国家自然科学基金资助项目（No 31570899）；河南省医学科技攻关计划项目 （）
