中国药理学通报2016，Vol.32Issue(6)：801-805,806,6.DOI:10.3969/j.issn.1001-1978.2016.06.013
BD NF-trkB信号通路在氯胺酮治疗糖尿病神经病理性疼痛中的作用
Role of BDNF-trkB signaling pathway in ketamine treating diabetic neuropathic pain
摘要
Abstract
Aim Toinvestigatetheroleofbrain-de-rived neurotrophic factor(BDNF)-tyrosine receptor ki-nase B (trkB ) signaling pathway in the therapeutic effects of ketamine on diabetic neuropathic pain.Meth-ods Forty-eightWistarrats,aged3months,weighing 200~250 g,were equally randomized into 4 groups(n=12 ):control group (C group ), saline group (S group),ketamine group (K group)and ketamine +ANA-12 group (KA group ).Rats in S,K and KA groups were intraperitoneally injected with a single of streptozotocin(STZ)65 mg·kg-1 to construct diabetic neuropathic pain model.After twenty-eight days,rats in S,K and KA groups were intraperitoneally injected with saline, ketamine 10 mg·kg-1 and ketamine 10 mg·kg-1 +ANA-12 0. 5 mg·kg-1 for consecutive 7 days, respectively. On the 8th day, mechanical withdrawal threshold(MWT)of rats was measured.Af-ter that,the rats were immediately sacrificed,and dor-sal ganglion of lumbar spine and prefrontal cortex (PFC)were harvested for measuring BDNF,p-trkB/trkB,synaptophysin and spine density by Western blot andglogistaining.Results ComparedwithCgroup, rats in S group significantly decreased MWT,BDNF, p-trkB/trkB,synaptophysin and spine density in dorsal ganglion and PFC (P <0. 05 ).Compared with S group,rats in K group showed a significant increase of MWT,BDNF,p-trkB/trkB,synaptophysin and spine density in the all observed regions(P<0. 05 ).On the contrary,rats in KA group showed a significant de-crease of MWT and BDNF,p-trkB/trkB,synaptophys-in and spine density as compared with K group in all regions(P<0. 05 ).Furthermore,BDNF was positive-ly correlated with spine density in all regions (P <0.05).Conclusion BDNF-trkBsignalingpathway mediates ketamine-induced therapeutic effects in dia-betic neuropathic pain.关键词
氯胺酮/神经病理性疼痛/脑源性神经营养因子/酪氨酸受体激酶/树突棘密度/突触素Key words
ketamine/neuropathic pain/brain-de-rived neurotrophic factor/tyrosine receptor kinase B/spine density/synaptophysin分类
医药卫生引用本文复制引用
宗剑,杨春,胡明珠,周波,季永..BD NF-trkB信号通路在氯胺酮治疗糖尿病神经病理性疼痛中的作用[J].中国药理学通报,2016,32(6):801-805,806,6.基金项目
无锡市医学管理中心医学科研面上项目 （）
