中国药理学通报2016，Vol.32Issue(6)：832-835,836,5.DOI:10.3969/j.issn.1001-1978.2016.06.018
钙／钙调蛋白依赖性蛋白激酶Ⅱ抑制剂K N-93加重大鼠离体心脏钙超载损伤
Ca2+/calmodulin-dependent protein kinase Ⅱ inhibitor K N-93 aggravates the calcium paradox-induced heart injury
摘要
Abstract
Aim ToinvestigatetheeffectsofCa2+/calmodulin-dependent protein kinase Ⅱ inhibitor KN-93 on calcium overload-induced heart injury.Methods Thirty-twoisolatedratheartswererandomlydivided into the control group,KN-93 control group,calcium paradox group,and calcium paradox with KN-93 treat-ment group.Left ventricular pressure were recorded, and the heart function was evaluated by the left ventric-ular end-diastolic pressure (LVEDP ) and developed pressure (LVDP).Coronary flow (CF)were collect-ed,and lactate dehydrogenase (LDH)content was de-termined.Triphenyltetrazolium chloride staining was usedtomeasuretheinfarctsize.Results Compared with the control group,KN-93 at 2. 5 μmol·L-1 had no effects on coronary flow,cardiac performance and cell death at the end of perfusion in normal rats (P>0. 05 );The hearts of calcium paradox exhibited a de-crease in LVDP and CF,meanwhile an increase in LV-EDP,LDH,and infarct size of 18 ±7. 2% (P <0. 01).2. 5 μmol·L-1 KN-93 further increased the levels of LVEDP,LDH and infarct size (P<0. 01)in Ca2+paradoxical hearts,while it provoked the decline intheCFandLVDP(P<0.01).Conclusion The data demonstrates that KN-93 aggravates heart injury in calcium paradox,it also suggests that CaMKⅡ is in-volved in the Ca2+overload-induced heart injury.关键词
钙超载/钙/钙调蛋白依赖性蛋白激酶Ⅱ/KN-9 3/钙离子/心脏/心肌损伤Key words
calciumoverload/CaMKⅡ/KN-93/calcium/heart/myocardial injury分类
医药卫生引用本文复制引用
孔令恒,顾晓明,南瑛,张建英,孙娜,朱娟霞,周京军..钙／钙调蛋白依赖性蛋白激酶Ⅱ抑制剂K N-93加重大鼠离体心脏钙超载损伤[J].中国药理学通报,2016,32(6):832-835,836,5.基金项目
国家自然科学基金资助项目（No．31371181）；陕西省科技厅项目（No 2014JM2-8164）；西安医学院学科建设经费资助 （）
