中国药理学通报2016，Vol.32Issue(6)：836-840,5.DOI:10.3969/j.issn.1001-1978.2016.06.019
毛蕊花糖苷通过激活PI3 K／AKT通路促进成年小鼠神经干细胞增殖
Acteoside promotes proliferation of neural stem cells from adult mice by activating PI3 K/AKT pathway
摘要
Abstract
Aim Toclarifytheeffectofacteosideon proliferation of neural stem cells (NSCs ) from adult mice,as well as the involved signaling pathway.Meth-ods NSCswereisolatedfromthesubventricularzone (SVZ)of adult C57BL/6 mice,then identified by im-munofluorescence staining with Nestin,the marker of NSCs.NSCs were exposed to acteoside (5,10,20,40μmol·L-1 )in absence of mitogen(EGF/bFGF)for 24 h.We employed CCK8 assay to detect NSCs viability and BrdU staining to identify NSCs proliferation.We performed Western blot to quantify the expression level ofp-AktinducedbyacteosideonNSCs.Results With-out mitogen,acteoside increased NSCs proliferation by activating p-Akt,which can be blocked by LY294002, the inhibitor of PI3K/AKT signaling pathway.Conclu-sion ActeosidepromotestheproliferationofNSCsfrom adult mice by activating PI3K/AKT pathway.关键词
毛蕊花糖苷/成年小鼠/神经干细胞/脑室下区/增殖/Akt通路Key words
acteoside/adult mice/neural stem cells (NSCs)/the subventricular zone(SVZ)/proliferation/Akt pathway分类
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林慧敏,段伟兵,邵瑞,韩立峰,朱彦,高秀梅,王彧..毛蕊花糖苷通过激活PI3 K／AKT通路促进成年小鼠神经干细胞增殖[J].中国药理学通报,2016,32(6):836-840,5.基金项目
天津市应用基础与前沿技术研究计划一般项目 （）
