中国药理学通报2016，Vol.32Issue(6)：858-862,5.DOI:10.3969/j.issn.1001-1978.2016.06.023
血根碱抑制人乳腺癌细胞MCF-7增殖的机制研究
Sanguinarine inhibits cell proliferation in MCF-7 human mammary adenocarcinoma cells
摘要
Abstract
Aim Toinvestigatewhetherexposureto Sanguinarine (SAN ) can inhibit cell proliferation in human mammary adenocarcinoma cells (MCF-7 ) and thepossiblemechanism.Methods WeexposedMCF-7 to anticancer compound SAN,cell viability was as-sessed by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ) reduction assay. ROS was measured using confocal microscopy,expres-sion of caspase-3 ,caspase-8 and caspase-9 were calcu-latedusingchemiluminescencemethod.Results SAN remarkably inhibited growth of human mammary adeno-carcinoma MCF-7 cells by decreasing cell proliferation. ROS release and caspase-3,caspase-8,caspase-9 ex-pression were stimulated by SAN in MCF-7 ,and these changes were abolished by the antioxidant,N-acetyl-cysteine(NAC).Conclusion Regulationofcaspases expression and release from MCF-7 cells are possibly e-voked by SAN through reactive oxygen species.关键词
血根碱/MCF-7/活性氧/caspase/凋亡/乳腺癌/增殖Key words
SAN/MCF-7/ROS/caspase/apoptosis/mammary adenocarcinoma/proliferation分类
医药卫生引用本文复制引用
路玉盼,董宪喆,冯霞,胡园,郑小丽,葛晓悦,汪进良,刘屏..血根碱抑制人乳腺癌细胞MCF-7增殖的机制研究[J].中国药理学通报,2016,32(6):858-862,5.基金项目
国家自然科学基金资助项目 （）
