中国药理学通报2016，Vol.32Issue(6)：868-872,873,6.DOI:10.3969/j.issn.1001-1978.2016.06.025
三黄汤对3 T3-L1脂肪细胞糖脂代谢的影响
Effect of Sanhuang Decoction on glucose and lipid metabolism in 3 T3-L1 adipocytes
摘要
Abstract
Aim ToobservetheeffectofSanHuang Decoction (SHD )on glucose and lipid metabolism in insulin resistance(IR)3T3-L1 adipocytes.Methods TheIRmodelof3T3-L1adipocyteswasinducedby high glucose and hyperinsulinism cultivation(also con-taining dexamethasone ).The adipocytes were treated with rosiglitazone(Ros)and different concentrations of SHD(2. 5,5,10,20,40 g·L-1 )for 24 h.The content of glucose disappeared from the culture medium was determined as glucose consumption of the cells. The transport of glucose was observed by 2-deoxidation-[3 H]-glucose uptake method.The efflux of nonesteri-fied fatty acids(NEFA)from adipocytes was observed by the concentration of NEFA in the culture medium. The mRNA expression of glucose transporter-4 (GLUT-4)was measured by real-time polymerase chain reac-tion (real-time PCR).The protein expression of GLUT-4wasdetectedbyWesternblot.Results Compared with the Con group,SHD(5,10,20,40 g·L-1 ) could significantly induce the glucose consumption and transportion(P<0. 01 ),increase the glycerol content in culture supernatant(P<0. 01 ),reduce the NEFA concentration in culture medium and accumulation of intracellular TG(P<0. 01 ).And all of these change showed a concentration-dependent effect when SFD concentrations were from 5 g·L-1 to 20 g·L-1 .At the same time,Ros could also remarkably induce the glucose consumption and transportion(P<0. 01 ),and increase the accumulation of intracellular TG (P <0. 01);but the concentration of NEFA and glycerol in culture medium did not show any difference in the Ros-treatedgroup(P>0.05).Conclusion SHDcanin-crease insulin sensitivity by increasing glucose trans-portation and consumption in the 3T3-L1 adipocytes as well as decreasing the NEFA efflux from the cells.关键词
三黄汤/葡萄糖/甘油三酯/甘油/游离脂肪酸/葡萄糖转运子4/3 T3-L 1 细胞Key words
Sanhuang Decoction/glucose/triglycer-ide/glycerol/free fatty acid/GLUT-4/3T3-L1 adipo-cytes分类
医药卫生引用本文复制引用
刘庆丰,刘毅,焦正,李中东,施孝金,钟明康..三黄汤对3 T3-L1脂肪细胞糖脂代谢的影响[J].中国药理学通报,2016,32(6):868-872,873,6.基金项目
上海市卫计委资助项目 （）
