中国药物经济学2016，Vol.11Issue(6)：18-20,3.DOI:10.12010/j.issn.1673-5846.2016.06.004
反相高效液相色谱法测定枯草杆菌二联活菌颗粒中甜菊糖苷的含量
Determination of Steviosin in Combine Bacillus Subtilis and Enterococcus Faecium Granules with Multivitamines,live By RP-HPLC
孙菲 1孙欣1
作者信息
- 1. 鸡西市食品药品检验检测中心，黑龙江鸡西 158100
- 折叠
摘要
Abstract
Objective To establish RP-HPLC method for determination of Steviosin in Combine Bacilus Subtilis and Enterococcus Faecium Granules with Multivitamines,live.Methods Using a column packed with AgiLent Zobax SB-C18 (250 mm×4.6 mm,5μm);The mobile phase was acetonitrile-0.002 mol/L H3pO4 by gradient elution and the detection wavelength was 210 nm;the flow rate was 1.0ml/min,the injection volume was 10μl,the column temperature was 30℃.Results The Linear response of Steviosin range from 5.160~103.2μg/ml r=0.9999(n=7);The average recovery of Steviosin was 99.04%,RSD= 0.6%(n=6).Conclusion The method is convenient and accurate and can be used for the quality control of Combine Bacilus Subtilis and Enterococcus Faecium Granules with Multivitamines,live.关键词
枯草杆菌二联活菌颗粒/含量测定/甜菊糖苷/反相高效液相色谱法Key words
Combine Bacilus Subtilis and Enterococcus Faecium Granules with Multivitamines,live/Determination/Steviosin/RP-HPLC分类
医药卫生引用本文复制引用
孙菲,孙欣..反相高效液相色谱法测定枯草杆菌二联活菌颗粒中甜菊糖苷的含量[J].中国药物经济学,2016,11(6):18-20,3.
