浙江医学2016，Vol.38Issue(12)：925-927,3.
山竹提取物对胃癌SGC-7901细胞的体外实验研究
Effect of Garcinia mangostana extract on proliferation of gastric cancer SGC-7901 cells in vitro
摘要
Abstract
Objective To investigate the effect of Garcinia mangostana Extract (GME) on proliferation of gastric cancer SGC- 7901 cells. Methods Human gastric cancer SGC- 7901 cel s were treated with 5μg/ml and 7.5μg/ml GME for 24h. SGC- 7901 cel proliferation was determined by tetrazolium salt reduction method (MTS), cel apoptosis and reactive oxygen species (ROS) were measured by flow cytometry, PTEN, Bax and Bcl- 2 protein expressions in SGC- 7901 cel s were detected by Western blot. Results GME significantly inhibited the proliferation of SGC- 7901 cells(P<0.05) with a 50%inhibitory concentrations of 7.89μg/ml, the inhibition rate was higher than that of 5- fluorouracil (5- FU)(P<0.05). After treated with 5μg/ml and 7.5μg/ml of GME for 24h, apoptosis of SGC- 7901 cel s was increased (P<0.05 ), and the cel ROS levels was also increased;the expressions of PTEN and Bax proteins in SGC- 7901 cel s were increased, while Bcl- 2 expression levels were reduced (P<0.05). Conclusion This study suggests that GME can significantly inhibit proliferation of SGC- 7901 cel s, enhance ROS levels, and promote apoptosis of SGC- 7901 cel s, which is associated with up- regulation of PTEN, Bax and down- regulation of Bcl- 2 expression.关键词
山竹提取物/增殖/胃癌/SGC- 7901细胞Key words
Garcinia mangostana extract/Proliferation/Gastric cancer/SGC- 7901 cel line引用本文复制引用
王荣国,李凯强,章志坚,王强..山竹提取物对胃癌SGC-7901细胞的体外实验研究[J].浙江医学,2016,38(12):925-927,3.基金项目
浙江省中医药优秀青年人才基金 （）
