安徽农业科学2016，Vol.44Issue(13)：135-137,219,4.
甘薯茎尖脱毒培养技术研究
Research on the Virus-free Culture Technique of Sweet Potato Stem Apical Meristem
陈玉霞 1邱建辉 1张朝臣 1刘作斌2
作者信息
- 1. 湖北省农业科学院农产品加工与核农技术研究所，湖北武汉430064
- 2. 湖北省农业科学院粮食作物研究所，湖北武汉430064
- 折叠
摘要
Abstract
[ Objective] The production of virus-free sweet potato plantlets by means of stem apical meristem-culturing and its identification method were researched. [ Method] To explore the technique of sweet potato stem apical meristem culturing, the single factor experiment in the sucrose and exogenous hormone added in MS medium was conducted. [ Result] The medium for plantlet regeneration was MS + BA 0. 5 mg/L + NAA 0. 10 mg/L + sucrose 40 g/L + agar 6. 5 g/L and the medium of plantlet rooting in vitro was 1/2MS+NAA 0. 20 mg/L su-crose 20 g/L+ agar 6. 5 g/L. 25 sweet potato varieties were cultured in the growth medium and the results showed that the rate of plantlet of 8 varieties was 50% -79. 3%;16 varieties, more than 80. 0% and 5 varieties, up to 100%. In the rooting medium, the rooting rate of plant-lets was 100% and the root system was strong and with more roots. The stem and leaf of plantlets vigorously grew. The plantlets could be di-rectly transplanted into fine sand and the survival rate of the plantlet reached 100%. The rate of virus-free in Brazil morning glory was 79. 1%with the technical system. [ Conclusion] The suitable media formulation for production of virus-free sweet potato plantlets and its simple identi-fication technique are provided.关键词
甘薯/茎尖/离体培养/脱毒/巴西牵牛Key words
Sweet potato/Stem apical/Culture in vitro/Virus-free/Brazil morning glory分类
农业科技引用本文复制引用
陈玉霞,邱建辉,张朝臣,刘作斌..甘薯茎尖脱毒培养技术研究[J].安徽农业科学,2016,44(13):135-137,219,4.
