安徽农业科学2016，Vol.44Issue(13)：149-152,170,5.
水热法制备磁性微球及其在植物基因组DNA提取分离中的应用
Preparation of Magnetic Nano-microsphere by Solvothermal Synthesis and Its Application in Genomic DNA Purification from Plant Tissues
摘要
Abstract
[ Objective] The aim was to establish a method for rapidly extracting genomic DNA from several kinds of plant tissues. [ Method] Fe3 O4 magnetic nano-microsphere was prepared by solvothermal synthesis, the surface features of particle size, morphology, magnetic proper-ties were analyzed. With Fe3 O4 magnetic nano-microsphere as nucleic acid extraction vector, DNA of leaves, roots, stems, grains and other tissues of several kinds of Xinjiang special economic crops and plants were extracted and seperated;links of purification and seperation process were optimized. [ Result] Through detection of OD260 UV absorption value, agarose gel electrophoresis, the results showed that the obtained genomic DNA with high purity and integrity can meet the requirements of the downstream molecular biology operation. [ Conclusion] A simple and rapid method for obtaining high yield and high quality genomic DNA from several kinds of plant tissues is established, which will provide theoretical and practical basis for automation and large-scale extraction of DNA.关键词
磁性纳米微球/水热法/基因组DNA/纯化/植物组织Key words
Magnetic nano-microsphere/Solvothermal synthesis/Genomic DNA/Purification/Plant tissues分类
医药卫生引用本文复制引用
杜鲜,孙培培,张虹,张富昌,石峰..水热法制备磁性微球及其在植物基因组DNA提取分离中的应用[J].安徽农业科学,2016,44(13):149-152,170,5.基金项目
石河子大学高层次人次科研启动项目( RCZX201138) （ RCZX201138）
石河子大学科学技术研究发展计划项目(2012ZRKYQ-YD14)。 （2012ZRKYQ-YD14）
