北京中医药大学学报2016，Vol.39Issue(6)：456-460,5.DOI:10.3969/j.issn.1006-2157.2016.06.004
防风多糖对升麻素在Caco-2细胞模型中吸收、转化的影响
Effect of polysaccharide in Radix saposhnikovae on absorption,and trans-formation of comofugin in Caco-2 cells
摘要
Abstract
Objective To study the influence of polysaccharide in Radix saposhnikovae(Divaricate Saposhnikovia Root,Fangfeng)on the absorption,and transformation of cimifugin in human small intestine.Methods Caco-2cell monolayer model was applied to stimulate thea bsorption mechanism of human small intestine.After detection of the contents of polysaccharide at apical side and at baso lateral side,the effect of polysaccharide on cimifugin absorption of small intestine was studied.The contents of total chromones were measured using HPLC after cimifugin co-cultured with Caco-2 cells for 2 h and 4 h. Results Concentration of polysaccharides made no difference on cimifugin permeation through Caco-2 monolayer cell membrane (P >0.05 ).After Caco-2 cells were cultured with polysaccharide and cimifugin solution for 2 h and 4 h,the content of polysaccharide had no effect on cimifugin content in culture solution and Caco-2 cells (P>0.05 ).But with the added quality of polysaccharide increasing, prim-O-glucosyl-cimifugin showed an increasing tendency,and 4′-O-β-glucosyl-5-O-methylvisamminol increased significantly(P<0.01),in direct proportion to quantity of polysaccharide.Cimifugin transfor-mation was due to polysaccharide,with the total chromones content of control group of 10.3 nmol/L and that of polysacchari degroup of 75.6 nmol/L.Conclusion Poly saccharide of Radixs aposhnikovaehasno obvious effect on the absorption of cimifugin,but can promote its transformation to prim-O-glucosyl-cimi-fugin and 4′-O-β-glucosyl-5-O-methylvisamminol,and prevent chromones from degradation.关键词
防风多糖/升麻素/色原酮/Caco-2细胞/吸收/转化Key words
Radix saposhnikovae/polysaccharide/cimifugin/Caco-2 cells/absorption/transformation分类
医药卫生引用本文复制引用
杨景明,姜华,王紫玮,胡立立,孟祥才..防风多糖对升麻素在Caco-2细胞模型中吸收、转化的影响[J].北京中医药大学学报,2016,39(6):456-460,5.基金项目
国家自然科学基金资助项目（No．81541079），辽宁省教育厅科学技术研究项目 （）
