北京中医药大学学报2016，Vol.39Issue(6)：461-465,5.DOI:10.3969/j.issn.1006-2157.2016.06.005
Notch蛋白在心肌梗死后大鼠心肌中的表达及益气活血方干预的研究
Expression of Notch protein in myocardium of acute myocardial infarc-tion rats and intervention of Yiqi Huoxue Formula
摘要
Abstract
Objective By establishing the acute myocardial infarction(AMI)model,toinvestigatethe expression of Notch signal protein in the myocardium of AMI model rats and the repair ment mechanism of Yiqi Huoxue Formula(supplementing qi and activating blood form ula)oncardiacinjury.Methods Al-together 64 SD rats,SPF grade,were randomly divided into sham group,model group,Yiqi Huoxue For-mula (TCM)group,perindopril group.The AMI model was induced by ligation of left coronary artery. Since the next day,the rats in TCM group and perindopril group were intragastrically (i.g.)adminis-tered corresponding drugs,with rats in sham group and model group administered distilled water.On Day 7 and Day 28,left ventricular ejection fraction (LVEF)and left ventricular fractional shortening (LVFS) were measured using visual sonic system designed exclusively for laboratory animals,pathological mor-phology was examined using HE staining,and the protein expressions of Notch 1 ,Notch 3 and Notch 4 weredetectedusing Westernblot.Results Comparedwithshamgroups,LVEF and LVFSinmodel he number of myocardial cells in the infarct border area decreased and inflammatory cells were observed.Notch 1 expression increased on both time points (P<0.01),Notch 3 expressed increasingly only on Day 7 (P<0.05).After interven-tion of TCM,LVEF,LVFS,Notch1 ,Notch3 decreased significantly compared with model group on Day 7 (P<0.05 ),LVEF increased significantly and Notch 1 expression decreased significantly on Day 28 (P<0.01).Conclusion Yiqi Huoxue Formula can improve cardiac function in rats with AMI,and lon-ger term application is more efficacy.Its biological mechanism maybe related to the injury related protein Notch 1 and Notch 3 .关键词
急性心肌梗死/益气活血方/损伤修复/信号通路/大鼠Key words
acute myocardial infarction/supplementing qi and activating blood formula/injury repair-ment/signal pathway/rats分类
医药卫生引用本文复制引用
杨丹丹,郭书文,孙晴,张璐,赵海滨..Notch蛋白在心肌梗死后大鼠心肌中的表达及益气活血方干预的研究[J].北京中医药大学学报,2016,39(6):461-465,5.基金项目
国家自然科学基金资助项目 （）
